17份蔷薇属植物的亲缘关系的形态学和ISSR分析  

Relationship of 17 Rosa Plants Detected by Morphology and ISSR Analysis

作　　者：杨帆[1] 曾丽[1] 叶康[2] 赵子刚[1] 张嫔[1] 龚霄雯[1] 尹勤 孙强 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海植物园,上海200231 [3]上海市林木总站,上海200072

出　　处：《植物研究》2011年第2期193-198,共6页Bulletin of Botanical Research

基　　金：上海市科委重大攻关项目(08DZ1906500);上海市农委重点攻关项目(2008第10-4号)

摘　　要：利用ISSR分子标记结合形态学指标对17份蔷薇属植物进行亲缘关系研究,采用NTSYS2.1进行聚类分析。结果表明:13个引物共扩增出479条带,其中有221条多态带,多态性比例为46.1%;根据形态学聚类分析,在相似系数为0.12时17份蔷薇属植物可分为三类,第一类包括‘宝岛玫瑰’、‘索菲之常花种’、‘凯拉伯爵夫人’、‘索菲的玫瑰’、‘藤神奇’、‘大富贵’、‘美人鱼’、‘金瓯泛绿’、‘金粉莲’和‘一季粉’;第二类包括‘绿萼’、‘湖中月’、‘羽士妆’、‘杨基歌’、‘四面镜’和‘匍匐红’;‘法国变色蔷薇’为第三类。根据ISSR聚类分析,在相似系数为0.66时可分为四类,第一类为‘宝岛玫瑰’;第二类为‘绿萼’、‘匍匐红’、‘凯拉伯爵夫人’、‘美人鱼’、‘四面镜’、‘湖中月’和‘羽士妆’;第三类为‘索菲的玫瑰’和‘杨基歌’;第四类为‘法国变色蔷薇’、‘金瓯泛绿’、‘藤神奇’、‘索菲之常花种’、‘一季粉’、‘大富贵’和‘金粉莲’。形态学聚类与ISSR聚类结果基本一致。The genetic relationship of 17 Rosa plants is analyzed by ISSR and morphological data. NTSYS2.1 is used in clustering analysis. The results showed： 13 primers were selected to perform ISSR -PCR. A total of 479 DNA bands was obtained, of which 221 bands had polymorphism, the percentage of polymorphic bands was 0. 46. All these plants could be divided into three groups by morphological data： the first group was consisted of Rosa rugosa ＇ Bao Dao＇ , Rosa chinensis ＇ Sophie＇ s perpetral＇ , Rosa chinensis ＇ Comtesse dll cayla＇ , Rosa hy- brid tea ＇ Sophy＇ s Rose＇ , Rosa hybrid climbingroses ＇ Ten Shen Qi＇ , Rosa chinensis ＇ Da Fu Gui＇ , Rose hybrid bracteata ＇Mermaid＇ , Rosa chinensis ＇Jin Ou Fan Lv＇ , Rosa chinensis ＇Jin Fen Lian＇ and Rosa chinensis ＇Yi Ji Fen＇ ; the second group was consisted of Rosa chinensis ＇ Viridiflora＇ , Rosa chinensis ＂ Hu Zhong Yue＇ , Rosa chinensis ＇ Yu Shi Zhuang＇ , Rosa hybrid tea ＇ Yankee Dodle＇ , Rosa chinensis ＇ Si Mian Jing＇ and Rosa chirten- sis ＇ Pu Fu Hong＇ ; the third group was consisted of Rosa gallica ＇ Versicolor＇. All these plants can be divided into four groups by ISSR cluster analysis. The first group was consisted of Rosa rugosa ＇ Bao Dao＇ ; the second group was consisted of Rosa chineasis ＇ Viridiflora＇ , Rosa chinensis ＇ Pu Fu Hong＇ , Rosa chinensis ＇ Comtesse dll cayla＇ , Rose hybrid bracteata ＇ Mermaid＇ , Rosa chinensis ＇ Si Mian Jing＇ , Rosa chinensis ＇ Hu Zhong Yue＇ and Rosa chinensis ＇ Yu Shi Zhuang＇ ; the third group was consisted of Rosa hybrid tea ＇ Sophy＇ s Rose＇ and Rosa hy- brid tea ＇ Yankee Dodle＇ ; and the fourth group was consisted of Rosa gallica ＇ Versicolor＇ , Rosa chinensis ＇ Jin Ou Fan Lv＇ , Rosa hybrid climbingroses ＇ Ten Shen Qi＇ , Rosa chinensis ＇ Sophie＇ s perpetral＇ , Rosa chinensis ＇ Yi Ji Fen＇ , Rosa chinensis ＇ Da Fu Gui＇ and Rosa chinensis ＇ Jin Fen Lian＇. The results of morphologic cluster analysis

关 键 词：蔷薇属 亲缘关系 简单重复序列区间扩增多态性(ISSR) 

分 类 号：S681.9[农业科学—观赏园艺]