外源H_2O_2处理对唐古特白刺愈伤组织脯氨酸代谢的影响  

Effect of Exogenous Hydrogen Peroxide on the Proline Metabolism in Nitraria tangutorum Bobr.Callus

作　　者：张园园[1] 吕秀军 张辉[1] 刘艳[1] 姜羽[1] 杨颖丽[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]甘肃省卫生学校,兰州730000

出　　处：《植物研究》2011年第2期213-217,226,共6页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(30960064);甘肃省自然科学研究基金(1010RJZA027);西北师范大学学生学术资助金

摘　　要：以唐古特白刺(Nitraria tangutorum Bobr.)愈伤组织为材料,研究外源H2O2(2和10μmol.L-1)处理下其脯氨酸含量及相关代谢酶活性的变化,试图从细胞水平揭示H2O2影响脯氨酸代谢的生理机制。结果显示,2和10μmol.L-1H2O2处理24 h使唐古特白刺愈伤组织脯氨酸含量分别变为对照的112%和92%,而处理72 h后,脯氨酸含量增加为对照的141%和119%;与对照相比,外源H2O2处理诱导愈伤组织脯氨酸脱氢酶活性降低,而谷氨酸激酶活性升高,但鸟氨酸转氨酶活性无显著变化;此外,H2O2处理使唐古特白刺愈伤组织内源性H2O2含量升高。结果表明,外源H2O2诱导了唐古特白刺愈伤组织H2O2含量的增高和脯氨酸的积累,且H2O2处理下脯氨酸脱氢酶活性的降低及谷氨酸激酶的升高与愈伤组织脯氨酸的积累有关。In order to understand the regulatory mechanism of proline metabolism at the cellular level, Nitraria tangutorum Bobr. callus was used to investigate the effects of exogenous hydrogen peroxide （ H2O2 ） treatment on the proline content and the activities of proline-related metabolism enzymes. After treatment with 2 and 10μmol·L-1 H2O2 for 24 h, proline content was about 112% and 92% of the control value in N. tangutorum Bobr. callus, respectively; at 72 h treatment, the amount of proline increased about 41% and 19% in comparison with the control. Analysis of enzyme activities showed that exogenous H2O2 induced remarkable decrease in proline dehydrogenase （ProDH） activity and significant increase in glutamyl kinase （GK） activity, but no significant changes in ornithine aminotransferase activity. In addition, the endogenous H2O2 level was increased in exoge- nous H2 O2-treated callus. In conclusion, our results indicated that exogenous H2O2 treatment resulted in the sig- nificant increase of endogenous H2O2 content in N. tangutorum Bobr. callus. Here it was also indicated that H2O2 induced proline accumulation, which was associated with the reduction of ProDH activity and the elevation of GK activity in N. tangutorum Bobr. callus.

关 键 词：唐古特白刺 愈伤组织 脯氨酸 过氧化氢 脯氨酸脱氢酶 

分 类 号：S727.23[农业科学—林木遗传育种]