弱光胁迫对大田长春花生物量分配及次生代谢的影响  

Effects of Low Light Stress on the Biomass Distribution and Secondary Metabolism of Catharanthus roseus

作　　者：佟璐[1] 张宝友[1] 唐中华[1] 郭晓瑞[1] 贾雪莹[1] 于景华[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2011年第2期227-230,240,共5页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(30770338)

摘　　要：以长春花(Catharanthus roseus(L.)Don.)为材料,研究了田间栽培长春花在全光和20%透光率下,经过一个生长季后生物量配置、抗氧化次生代谢产物和文朵灵、长春质碱、长春碱等目的活性物质含量的变化。研究结果表明,弱光条件显著抑制了长春花植株总生物量增长,特别是抑制了有性生殖的投入;弱光组叶片总酚和总黄酮含量显著降低,干重含量分别为对照组的62.50%和50.00%,原花青素含量则略有升高,但与全光组的差异不显著;弱光组叶片文朵灵和长春质碱含量显著高于对照组,长春碱含量略有上升但差异不显著,受生物量降低影响,3种生物碱的产量均显著下降。上述结果表明,长春花能够调控生理代谢以适应低光强环境,特别是文朵灵和长春质碱含量提升显著,林下低光强环境种植长春花可以满足土地资源充分利用和文朵灵、长春质碱优质原料的需求。Under full exposure and 20% exposure to light, the field-cultivated Catharanthus roseus （ L. ） Don. was used to investigate the changes of biomass distribution, antioxidative secondary metabolites and contents of objective active products such as vindolin, catharanthine and vinblastine after a growing season. The results indicated that the total biomass of C. roseus was extremely restrained by the low light intensity, especially the investment of generative propagation; the contents of total phenolies and total flavonoids in leaves under low light intensity decreased distinctly, and the contents of dry weight were 62.50% and 50.00% of the control, respectively, while the content of proanthocyanidins increased slightly, but not remarkably as compared to the full exposure group; vindo- fin and catharanthine contents under low light intensity were much higher than that of control while vinblastine con- tent increased slightly; the contents of three alkaloids all reduced, which was affected by the reduction of biomass. In conclusion, C. roseus could regulate physiological metabolism to adapt the low light environment, including the remarkable increased content of vindolin and eatharanthine. Land resource was fully utilized by planting C. roseus in low light environment. Meanwhile, the needs of vindolin and row material of vinblastine were satisfied as well.

关 键 词：长春花 弱光胁迫 生物量分配 目的活性物质 立体种植 

分 类 号：S567[农业科学—中草药栽培]