免疫球蛋白对心梗后心衰大鼠血清IL-6、TNF-α表达的影响  

Effect of immunoglobulin on the expressions of IL-6,TNF-α in heart failure rats after acute myocardial infarction

作　　者：杨杰[1] 杨萍[2] 怀淑君[3] 

机构地区：[1]吉林大学中日联谊医院内分泌科,吉林长春130033 [2]吉林大学中日联谊医院心内科,吉林长春130033 [3]吉林大学中日联谊医院急诊科,吉林长春130033

出　　处：《中国老年学杂志》2011年第5期790-792,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.130770883)

摘　　要：目的探讨免疫球蛋白对心梗后心衰大鼠IL-6、TNF-α及血浆B型钠尿肽(BNP)水平的影响及其改善心功能的机制。方法取雄性Wistar大鼠30只,随机取8只作为假手术组(n=8),另外22只通过持续结扎前降支冠状动脉建立大鼠动物模型,ELISA法测血清中心脏肌钙蛋白T(CTnT)水平,将CTnT水平>4.00 ng/ml的15只大鼠,随机分为模型组(n=7)及免疫球蛋白组(n=8),喂养6 w后,免疫球蛋白组腹腔注射给予免疫球蛋白400 mg.kg-1.d-1,非给药组均腹腔注射给予0.9%生理盐水,连续给药5 w后测定大鼠血流动力学参数,用ELISA方法检测血清中IL-6、TNF-α及BNP水平。结果与假手术组比较,模型组大鼠血清IL-6、TNF-α水平呈高水平表达,具有显著差异(P<0.01),同时BNP的蛋白表达也明显增高(P<0.01)。免疫球蛋白组IL-6、TNF-α及BNP水平明显低于模型组(P<0.01)。结论免疫球蛋白可以减少心肌梗死后大鼠血清中炎症因子的表达,并降低血浆中BNP水平,在一定程度上起到改善心脏功能的作用。Objective To investigate the effects of immunoglobulin（IVIG） on the levels of IL-6,TNF-α and BNP in heart failure rats after acute myocardial infarction（AMI）.Methods The rat models of heart failure were established by left anterior descending coronary artery deligation.After 6 w,15 rats were divided into model and IVIG groups at random,in addition,sham-operated group was selected,and were treated with medicines by intraperitoneal injection respectively.At 11 w,the levels of serum IL-6,TNF-α and BNP were observed.Results The levels of IL-6,TNF-α and BNP of model groups were increased significantly,IVIG decreased significantly the levels of IL-6,TNF-α and BNP.Conclusions IVIG can reduce the contents of inflammatory factors in heart failure rats after AM I,it also reduce the content of BNP,which may be related to improving heart function.

关 键 词：免疫球蛋白 白细胞介素-6 肿瘤坏死因子-α 心脏肌钙蛋白T B型钠尿肽 

分 类 号：R543.3[医药卫生—心血管疾病]