高糖环境下VEGF与PEDF在GMC中表达及rAAV-AS基因的干预作用  

Expression of pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor (VEGF) in cultured glomerular mesangial cells (GMC) by high glucose and interventional effects of rAAV-AS

作　　者：赵猛[1] 李伟[1] 

机构地区：[1]徐州医学院附属医院,江苏徐州221002

出　　处：《中国老年学杂志》2011年第5期818-820,共3页Chinese Journal of Gerontology

基　　金：江苏省高校自然科学基金资助项目(No.06KJB320117)

摘　　要：目的探讨高糖对大鼠肾小球系膜细胞(GMC)色素上皮细胞衍生因子(PEDF)和血管内皮生长因子(VEGF)表达影响以及腺相关病毒介导血管抑素(rAAV-AS)基因的干预作用。方法将培养的大鼠GMC株分为6组,高糖作为刺激因素,rAAV-AS作为干预因素。分别设低糖组、高糖组、高糖加腺相关病毒(rAAV)组及高糖加rAAV-AS治疗组。用免疫细胞化学及ELISA法测定各组系膜细胞PEDF以及VEGF蛋白表达。结果高糖可下调GMC中PEDF蛋白的表达,上调GMC中VEGF蛋白的表达。rAAV-AS可逆转高糖导致GMC的VEGF蛋白表达的增加及PEDF蛋白表达的降低。结论 rAAV-AS可以一定程度改善高糖诱导的VEGF和PEDF表达失衡而发挥对糖尿病肾病(DN)的保护作用。Objective To explore the effect of pigment epithelium-derived factor（PEDF） and vascular endothelial growth factor（VEGF） in cultured glomerular mesangial cells（GMCs） and intervention of recombinant adeno-associated virus mediated angiostatin gene.Methods Cultured HBZY21 rat GMCs were divided into 6 groups（glucose as a stimulated factor,rAAV-AS as an intervenor）： low glucose,high glucose,high glucose and rAAV,high glucose and rAAV-AS.The expressions of PEDF and VEGF of each group were measured by immunohistochemistry and ELISA.Results（1）The level of VEGF protein was remarkably increased and the level of PEDF protein was decreased by high glucose.（2） High glucose could increase the expression of VEGF and decrease significantly the expression of PEDF.rAAV-AS could prevent this change induced by high glucose.Conclusions rAAV-AS might play an important role in preventing diabetes nephropathy by partly compensating the unbalanced expressions of VEGF and PEDF induced by hyperglycemia.

关 键 词：糖尿病肾病 色素上皮细胞衍生因子 血管内皮生长因子 血管抑素 

分 类 号：R692[医药卫生—泌尿科学]