LHRHa靶向微泡造影剂的制备及体外寻靶实验  

Preparation of LHRHa-targeted microbubbles contrast agent and targeting study in vitro

作　　者：郭娟[1] 常淑芳[1] 孙江川[1] 严宇[1] 廖永玲[1] 王志刚[2] 

机构地区：[1]重庆医科大学附属第二医院妇产科,重庆400010 [2]重庆医科大学超声影像研究所,重庆400010

出　　处：《中国医学影像技术》2011年第3期466-469,共4页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金(30801228);重庆市自然科学基金(CSTC;2008BB5405);重庆市卫生局项目(07-2-098)

摘　　要：目的制备人卵巢癌靶向超声造影剂,观察其体外寻靶能力。方法采用生物素-链霉亲和素法制备促黄体生成素释放激素类似物(LHRHa)靶向脂质微泡,以免疫荧光染色验证LHRHa与脂质微泡的结合情况,并以普通脂质微泡作为对照,在倒置显微镜下观察LHRHa靶向脂质微泡对人卵巢癌A2780/DDP的体外寻靶能力。结果 LHRHa靶向脂质微泡免疫荧光阳性;体外寻靶实验显示LHRHa靶向脂质微泡能够与表面存在LHRH受体的A2780/DDP细胞特异性结合,而普通脂质微泡则不能结合。结论利用生物素-链霉亲和素方法可成功制备LHRHa靶向脂质微泡造影剂,该靶向造影剂具有体外寻靶能力。Objective To prepare targeted microbubbles targeting ovarian cancer cells,and to assess its targeting ability in vitro.Methods The biotinylated luteinizing hormone releasing hormone analog（LHRHa） peptides were conjugated to the biotinylated microbubbles via streptavidin,and then were observed with fluorescence microscope.The LHRHa carrying microbuibbles were added into A2780/DDP cells to observe the adherence of the targeted microbubbles to the cells,and normal microbubbles were used as control.Results Fluorescence was expressed on the targeted microbubbles shell in vitro.The LHRHa carrying microbubles could adhere to the A2780/DDP cells specifically,whereas there was no adherence of normal microbubbles to the A2780/DDP cells.Conclusion The LHRHa carrying microbubbles having targeting ability in vitro can be successfully constructed via biotin-sterptavdin binding.

关 键 词：微泡 生物素-链霉亲和素复合体 促性腺激素释放激素 卵巢肿瘤 

分 类 号：R445.1[医药卫生—影像医学与核医学]