CTLA4-Ig基因修饰的树突状细胞体外对Th1/Th2平衡的影响  

The effect of CTLA4-Ig gene modified dendritic cell on Th1/Th2 balance in vitro

作　　者：应林燕[1,2] 符州[1] 罗健[1] 陈艳[3] 刘恩梅[1] 王莉佳[2] 

机构地区：[1]重庆医科大学附属儿童医院呼吸中心 [2]儿童发育疾病研究省部共建教育部重点实验室,重庆400014 [3]上海复旦大学附属儿科医院,上海201102

出　　处：《细胞与分子免疫学杂志》2011年第3期266-269,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30600684)

摘　　要：目的:探讨细胞毒性T淋巴细胞相关抗原4免疫球蛋白(CTLA4 Ig)基因修饰的树突状细胞(CTLA4 Ig-DCs)体外对Th1/Th2平衡的影响。方法:通过腺病毒载体将目的基因(CTLA4 Ig)转染至小鼠骨髓来源的树突状细胞(DC)。采用流式细胞术(FCM)检测DC表面分子和胞内CTLA4 Ig的表达;采用混合淋巴细胞反应检测DC刺激同种异体T细胞的能力,ELISA法检测DC抗原提呈反应中Th1和Th2类细胞因子IFN-γ和IL-4分泌水平。结果:CTLA4 Ig基因成功转染至DC,转染率约为80%,制备的CTLA4 Ig-DCs稳定表达CTLA4Ig,表面分子CD86呈现低表达;CTLA4 Ig-DCs可有效抑制T细胞增殖,降低抗原提呈反应上清中IFN-γ和IL-4的分泌,并增加IFN-γ/IL-4比值。结论:通过腺病毒将CTLA4 Ig转染DC并且高效表达,可有效降低DC表面CD86分子,抑制同种异体T细胞反应,并能影响体外Th1/Th2水平。AIM： To investigate the impact on Th1/Th2 subset balance from cytotoxic T lymphocyte-associated antigen immunoglobulin（CTLA4 Ig） gene-modified dendritic cells（CTLA4 Ig-DCs） in vitro.METHODS： The modified DCs（CTIA4 Ig-DCs） were prepared by transferring the mouse bone marrow-derived DCs with the constructed adenovirus CTIA4 Ig vector.The CTLA4 Ig expression and certain cell surface molecules on the CTIA4Ig-DCs were detected by FCM,the potential to stimulate allogeneic T cell proliferation of the modified DCs by mixed lymphocyte reaction（MLR） and the secretion of IFN-γ and IL-4 in antigen presentation by ELISA.RESULTS： CTLA4 Ig gene was successfully transfected into DCs with stable expression of CTLA4 Ig,and its transfection efficiency was about 80%.Compared with the controls,CTLA4 Ig-DCs showed lower surface molecule CD86,inhibited allogeneic lymphocyte proliferation in MLR,decreased secretion of IFN-γ and IL-4 and increased ratio of IFN-γ/IL-4 in antigen presentation.CONCLUSION： CTLA4 Ig-DCs were successfully obtained and effectively expressed CTLA4 Ig,which could reduce the expression of CD86 on DCs surface.CTLA4 Ig-DCs inhibited allogeneic T cell proliferation and affected the ratio of Th1/Th2 in vitro.

关 键 词：树突状细胞 CTLA4IG TH1/TH2平衡 

分 类 号：R392.8[医药卫生—免疫学]