IL-21在不同乙型肝炎病毒感染者外周血CD4^+T细胞中表达的初步研究  

The induced IL-21 levels in CD4^+ T cells of peripheral blood from the different clinical types of patients infected with hepatitis B virus

作　　者：丁艳荣[1] 张野[1] 连建奇[1] 李新红[1] 王临旭[1] 黄长形[1] 

机构地区：[1]第四军医大学唐都医院传染科,陕西西安710038

出　　处：《细胞与分子免疫学杂志》2011年第3期304-306,308,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(81072434)

摘　　要：目的:探讨乙肝病毒感染不同临床表现患者外周血CD4+T细胞中IL-21表达的差异及其在发病机制中的作用。方法:分离乙肝病毒感染不同临床表现患者及健康人外周血单个核细胞(PBMC),使用PMA+ionomycin进行刺激,同时每份标本未加PMA+ionomycin刺激做阴性对照,流式细胞术(FCM)检测IL-21的表达情况及其与Th17细胞亚群的关系。结果:PMA+ionomycin刺激能够诱导IL-21的产生,产生IL-21的主要为CD4+T细胞,IL-17A+IL-21+CD4+T细胞几乎检测不到,IL-21+CD4+T细胞比例在急性乙型肝炎组、乙肝病毒携带组中较正常对照组和慢重肝组有所升高,Th17细胞亚群比例在各组中没有统计学差异;除急性乙型肝炎组外,其余各组中IL-21+CD4+T细胞比例与Th17细胞亚群比例均有较好的相关性。结论:IL-21在HBV感染不同临床表现患者外周血CD4+T的表达有一定差异,并且其与Th17细胞亚群有相关性,提示IL-21在HBV感染的发病机制可能发挥一定作用。AIM： To investigate the levels of interleukin（IL）-21 in CD4＋T cells of peripheral blood from the different types of patients infected with hepatitis B virus（HBV） and elucidate its role in the hepatitis B pathogenesis.METHODS： Peripheral blood mononuclear cells（PBMCs） from the patients infected with HBV and healthy individuals were stimulated with or without PMA coupled with ionomycin.The levels of IL-21 in CD4＋T cells and Th17 cells were analyzed by flow cytometry.RESULTS： PMA and ionomycin induced the expression of IL-21,and IL-21 was mainly produced by CD4＋ T cells,but IL-17A＋IL-21 ＋CD4 ＋ T cells were not detected.The frequencies of IL-21＋CD4＋ T cells in the patients of acute hepatitis B and chronic asymptomatic HBV carriers were higher than in healthy controls and severe chronic hepatitis B patients;there were no remarkable differences in the proportion of Th17 cells among the different groups of patients.Furthermore,the proportion of IL-21＋CD4＋ T cells correlated with Th17 cells in all groups except for the acute hepatitis B patients.CONCLUSION： IL-21 levels,which correlated with Th17 cells,were different in CD4＋T cells from the different types of patients infected with hepatitis B virus（HBV）.The results showed that IL-21 may play a role in the hepatitis B pathogenesis.

关 键 词：IL-21 HBV感染患者 TH17细胞 

分 类 号：R512.6[医药卫生—内科学]