难治性癫痫细胞模型中神经元损伤及神经突起的变化  

Neuronal damage and neurite change in cell model of intractable epilepsy

作　　者：吴原[1] 刘秀颖[1] 王学峰[2] 唐玉兰[1] 余璐[1] 苏婕[1] 吴月娟[1] 马美刚[1] 

机构地区：[1]广西医科大学第一附属医院神经内科,南宁530021 [2]重庆医科大学一附院神经内科

出　　处：《中华神经科杂志》2011年第3期196-199,共4页Chinese Journal of Neurology

基　　金：基金项目：国家自然科学基金资助项目（30960111）;广西自然科学基金资助项目（2010GXNSFA013168）

摘　　要：目的 了解难治性癫痫细胞模型神经元损伤情况及神经突起的形态学变化.方法 培养至第10天的海马神经元用无镁液处理3 h,制备成难治性癫痫细胞模型,测定乳酸脱氢酶（lactic acid dehydrogenase,LDH）释放量了解神经元损伤情况,应用光学显微镜及扫描电镜观察神经元和神经突起的形态学变化.结果 模型组LDH释放量（U/L）在无镁液处理后3、6、24 h（分别为4.26±1.28、6.56±2.34和16.67±3.57）均较对照组明显升高（LSD法,P〈0.05）.随着时间的延长,模型组LDH释放量逐渐升高（F=39.316,P〈0.05）.经无镁液处理后24 h,光学显微镜下可见神经元胞体相互靠近,神经突起相互迁移聚集,突起连接成网格样变化,72 h时这种变化更加明显.扫描电镜下可见神经元胞膜粗糙,有小凹陷形成,神经突起交织聚集.结论 难治性癫痫细胞模型存在神经元损伤,存在神经突起的形态学变化.Objective To establish the cell model of intractable epilepsy and to observe its neuronal damage and morphologic change of neurites.Methods The model was established by exposing hippocampal neurons to Mg2＋ -free media for 3 hours on days 10 of culture.Expression of lactic acid dehydrogenase （LDH） in supernatant was measured as an index of neuronal damage.The morphologic change of neurons and neurites was observed by optical microscope and scanning electron microscope （SEM）.Results Compared to the control group, level of LDH （U/L） was significantly increased in the model group at different time points （3 hours： 4.26 ± 1.28, 6 hours： 6.56 ±2.34 and 24 hours： 16.67 ±3.57, P 〈0.05）.With time prolonging, release of LDH in the model group was gradually increased （F = 39.316,P 〈0.05）.Under optical microscope, neurons of model group migrated closely to each other and neurite connections appeared to be gradually ＂reticulated＂ after Mg2＋ -free media treatment for 24 hours; and the ＂reticulated＂ neurites connections become more obvious after 72 hours.Under SEM, neuronal membrane was rough and had several small depressions, neurites were interlaced in cluster.Conclusions Neuronal damage and morphologic change of neurites are verified in the cell model of intractable epilepsy.

关 键 词：癫痫 神经元 神经突 模型 神经学 

分 类 号：R285.5[医药卫生—中药学]