黑龙江上游3种茴鱼分类地位的线粒体D-loop序列变异分析  

Taxonomic status of three fish species in Thymallus from upper Heilongjiang River based on mitochondrial control region sequence variation

作　　者：马波[1] 孙家贤[2] 姜作发[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所农业部黑龙江流域渔业资源环境重点野外科学观测试验站,黑龙江哈尔滨150070 [2]东北农业大学水产养殖系,黑龙江哈尔滨150030

出　　处：《中国水产科学》2011年第2期314-321,共8页Journal of Fishery Sciences of China

基　　金：科技部社会公益性专项基金资助项目(2002DIB5012);中央级公益性科研院所基本科研业务费专项资金项目(2007HSYZX-ZH-24)

摘　　要：基于茴鱼属(Thymallus)鱼类的线粒体D-loop片段全序列变异,对分布于黑龙江上游中国境内的呼玛河、额木尔河等水系中形态特征差异显著且同域分布的3种茴鱼共24个个体进行分子系统发育关系研究,以探讨其在属内的分类学地位。结果显示,分布于黑龙江上游的茴鱼群体在属内显著分为3个独立进化分支,平均序列分歧为0.048～0.062,已达到种间分化水平。经形态学鉴定,其中2个分支分别为黑龙江茴鱼(T.grubii)和下游黑龙江茴鱼(T.tugarinae),而另外1个分支应为1个新种;且该种与分布于勒拿河上游的勒拿河茴鱼(T.sp.)构成单系群,平均序列分歧仅为0.006,即黑龙江上游和勒拿河上游的待定名茴鱼为同一种,结合文献,该茴鱼被确定为北极茴鱼(T.arcticus)的1个亚种。综合3种茴鱼在黑龙江水系的地理分布现状,对其起源及演化进行了初步推测。Three sympatric species in the genus Thymallus with distinguishable morphological characters in upper Heilongjiang River of China were sampled from Huma River,Emur River and other branches of upper Heilongjiang River.Twenty-four complete nucleotide control region of the mtDNA were sequenced and the phylogenetic tree was constructed for their taxonomic status.The results showed that the Thymallus fishes in upper Heilongjiang River were divided into 3 monophyletic groups,and the mean genetic difference was 0.048-0.062,which showed genetic difference among species.The results imply there are 3 species of Thymallus in upper Heilongjiang Rive.According to morphological data,2 of them were T.grubii and T.tugarinae,respectively,and another one should be a new record species of Heilongjiang River,which clustered together with the grayling in upper Lena River,and mean genetic diversity was 0.006 and made up of a sister group with T.pallasii and T.baicalensis,between which the mean genetic diversity was 0.029-0.038.Based on this study,the conclusion is that there are 3 species in Thymallus including T.grubii,T.tugarinae and a new record one.of which the origin and evolution with their distribution are discussed in this study.

关 键 词：茴鱼属 D-loop序列 分类地位 系统发育 黑龙江上游 

分 类 号：Q961[生物学—昆虫学]