β-环糊精聚合物—二茂铁安培型葡萄糖氧化酶电极  

An Amperometric Enzyme Electrode for Glucose by Immobilizing Glucose Oxidase and Ferrocene in β-Cyclodextrin

作　　者：刘盛辉[1] 莫卫民[1] 陈帆[2] 

机构地区：[1]浙江工业大学化学工程学院,杭州310014 [2]温州师范学院化学系,温州320003

出　　处：《分析测试学报》1999年第4期42-45,共4页Journal of Instrumental Analysis

摘　　要：利用 β-环糊精的空穴结构 ,通过主客体化学反应将二茂铁包络在 β-环糊精聚合物的空穴中 ,同时将葡萄糖氧化酶交联在 β-环糊精聚合物上 ,制成对葡萄糖有灵敏响应的生物传感器。循环伏安和安培检测表明包络在 β-环糊精聚合物空穴中的二茂铁更加接近酶的氧化还原中心 ,可以有效地作为葡萄糖氧化酶和玻碳电极之间的电子媒介体 ,葡萄糖浓度在1.0×10-2 ～8.0mmol/L范围内 ,其浓度与电流响应值呈良好的线性关系 ,方法的检出限为2.0×10-3 mmol/L(S/N=3) ,在10s之内达到最大响应的95 %。用该法测定了血清中的葡萄糖含量 ,结果与传统方法吻合。A simple and sensitive biosensor for glucose has been developed by immobilizing glucose oxidase in β _ cyclodextrin via cross _ linking and by including ferrocene in the cavities of dextrin polymer via host _ guest reaction. Cyclic voltammetry and amperometric measurements indicated a better efficacy of electron transfer between immobilized glucose oxidase and the glassy carbon electrode owing to the closer proximity of the included ferrocene to the redox center of the enzyme. Under optimal conditions, the response of the sensor to glucose is linear in the concentration range of 1.0×10-2 ～ 8.0 mmol·L-1. The detection limit is 2.0×10-3 mmol·L-1(S/N=3). The time required to reach 95% of the maximum response is less than 10 s. Ascorbic acid and uric acid do not interfere on the detection of glucose. The results of glucose determination in serum samples agree with conventional methods.

关 键 词：环糊精聚合物 二茂铁 葡萄糖氧化酶 传感器 

分 类 号：O629.112[理学—有机化学] O657.1[理学—化学]