用反相高效液相色谱法测定小鼠心肌、骨骼肌中ATP、ADP和AMP的含量  

Determination of ATP, ADP and AMP in Rat Myocardium and Skeletal Muscles by RP-HPLC

作　　者：吴珏珩[1] 汤丽芬[1] 谭炳炎[1] 欧阳彬[2] 李章旺 

机构地区：[1]中山医科大学 [2]中山医科大学第一附属医院,广州510089

出　　处：《分析测试学报》1999年第4期55-57,共3页Journal of Instrumental Analysis

摘　　要：采用反相高效液相色谱法同时测定小鼠心肌、骨骼肌组织中ATP(三磷酸腺苷 )、ADP(二磷酸腺苷 )、AMP(一磷酸腺苷 )的含量。以150mmol/L磷酸二氢钾缓冲液 (pH=6.25)为流动相 ,采用BDShypersilC18 不锈钢色谱柱 ,紫外检测波长为254nm ,线性范围为1～200mg/L。ATP、ADP和AMP的平均加标回收率均在90 %以上 ,检出限分别为10ng、10ng、15ng。该法灵敏、准确 ,试剂费用低。The content of ATP,ADP and AMP in both rat myocardium and skeletal muscles were determined by RP-HPLC with a BDS C18 column(5 μm, ID 4.6 mm×30 cm ), and UV detector at a wavelength of 246 nm. Mobile phase was 150 mmol/L KH2PO4(pH=6.25). All the calibration curves are linear in the mass concentration range of 1～200 mg/L. The average recoveries of the standard additions are over 90%, and detection limits are 10 ng, 10 ng and 15 ng for ATP, ADP and AMP, respectively.

关 键 词：ATP ADP AMP 心肌 骨骼肌 测定 HPLC 

分 类 号：Q493.8[生物学—生理学] Q524