罗格列酮对兔急性心肌梗死后心室重构及心功能的影响  

Effect of Rosiglitazone on the ventricular remodeling and cardiac function of rabbit with acute myocardial infarction

作　　者：王晓丽[1] 柳万千[2] 周红丽[3] 

机构地区：[1]辽宁医学院附属第一医院老年医学科,辽宁锦州121001 [2]江西省九江市第一人民医院心内科,江西九江330300 [3]辽宁医学院附属第一医院肾内科,辽宁锦州121001

出　　处：《解剖科学进展》2011年第2期154-156,160,共4页Progress of Anatomical Sciences

基　　金：辽宁省教育厅资助项目(No.2009A458)

摘　　要：目的探讨罗格列酮(Ros)对兔急性心肌梗死(AMI)后心室重构和心功能的影响。方法将30只日本大耳白兔随机分为:假手术(Sham)组,AMI模型组,Ros组,每组10只。永久性结扎冠状动脉左前降支中上1/3处制作AMI模型,术后6hRos组给予Ros3mg/(kg·d)灌胃,AMI组及sham组给予等量蒸馏水灌胃。4周后心脏超声检查,测量左室收缩末期内径(LVESD)、左室舒张末期内径(LVEDD)、左室射血分数(EF)及短轴缩短率(FS)。用电子天平称取左心室重量(游离壁+室间隔),计算左室重量指数(左心室重量/体积质量),放射免疫法测肿瘤坏死因子α(TNF-α)变化。结果经Ros处理4周后,AMI组兔LVESD和LVEDD明显高于sham组,而EF和FS明显低于sham组(<0.01)。Ros组兔LVESD和LVEDD,左心室重量和左心室重量指数,心肌匀浆TNF-α水平显著低于AMI组(<0.01),EF显著高于AMI组(<0.01)。HE染色示AMI组心肌细胞纤维化明显,大量炎性细胞浸润,Ros组纤维化及炎性细胞浸润减少。结论 Ros抑制兔AMI后心室重构,改善心脏功能,可能与其抑制促炎症因子有关。Objective To evaluate the effect of Rosiglitazone on rabbit ventricular remodeling and cardiac function after acute myocardial infarction（AMI） . Methods Thirty rabbits were randomly divided into：sham operation group（Sham） ,acute myocardial infarction（AMI） group,Rosiglitazone（Ros） group averagely. AMI model was established by ligation of the left anterior branches of the coronary artery,rabbits in Ros group were treated with Rosiglitazone 3mg/（ kg·d ） by direct gastric gavage 6 hs after surgery,while AMI group and sham group were treated with equal amount of distille water. Left ventricular end-systolic dimension（LVESD） ,left ventricular end-diastolic dimension（LVEDD） ,ejection fraction（EF） ,shortening fraction（FS） were measured by heart ultrasound 4 weeks after surgery. Left ventricular weight（free wall＋ interventricular septum） ,left ventricular weight index（left ventricle weight/volume quality） were examined with an electronic scale,level of tumor necrosis factor α（TNF-α） was determined by radioimmunoassay. Results The values of LVESD and LVEDD were higher,but the values of EF and FS were lower in AMI group than in sham group. The values of LVESD,LVEDD,Left ventricle weight,left ventricle weight index,and level of TNF-α in myocardium homogenate were significantly lower in Ros group than in AMI group,whereas EF was higher in Ros group than in AMI group. HE staining showed that the extent of myocardial cells fibration and inflammatory cells infiltration increased in AMI group,but decreased in Ros group. Conclusion Rosiglitazone can inhibit the ventricular remodeling and improve cardiac function after AMI,which may be related to the inhibition of the expression of inflammatory factors.

关 键 词：罗格列酮 急性心肌梗死 心室重构 心功能 兔 

分 类 号：R541.4[医药卫生—心血管疾病]