成年恒河猴大脑皮质BDNF分离、鉴定及其分布  

Isolation and Identification of BDNF in Cortex of Adult Rhesus Monkey and Its Distribution

作　　者：李玉[1] 王波[1] 王廷华[2] 习杨彦彬[2] 

机构地区：[1]昆明医学院第一附属医院神经外科,云南昆明650032 [2]昆明医学院神经科学研究所,云南昆明650031

出　　处：《昆明医学院学报》2011年第1期26-29,共4页Journal of Kunming Medical College

基　　金：美国纽约中华医学基金资助项目(CMB-OO-72)

摘　　要：目的分离、鉴定成年恒河猴大脑皮质BDNF,并探讨其组织分布.方法取成年恒河猴大脑皮质组织,匀浆制作蛋白提取液,SDS-PAGE电泳分离,Western blot鉴定.进而制作猴大脑皮质冰冻切片,用BDNF抗体行免疫组织化学SP法染色,观察BDNF免疫阳性反应在成年恒河猴大脑皮质的分布,探讨BDNF的生物学功能.结果电泳分离后,Western blot检测到分子量14 kD左右有BDNF特异阳性染色条带.免疫组化染色显示猴脑皮质第III和第V层神经元胞浆和突起均有BDNF染色.结论电泳可分离恒河猴大脑皮质多种蛋白质,其中BDNF能通过Western blot识别鉴定;BDNF主要定位猴脑皮质神经元,提示其在灵长类皮质神经元功能维持中的重要作用.Objective To isolate and identify brain derived neurotrophic factor（BDNF）in the cerebral cortex of adult Rhesus monkey,and investigate its histological distribution.Methods The cortex tissues of monkeys were harvested,homogenized and isolated by SDS PAGE electrophoresis,and identified by western blot using rabbit BDNF specific antibody.Then the frozen sections from cortex were prepared,and the immunohistochemistry method was used to detect the distribution of BDNF in the cerebral cortex of adult Rhesus monkey.The biological function of BDNF was discussed.Results After electrophoresis,Western blot showed that a specific band for BDNF located in 14KD molecule weight was recognized from the homogenization of monkey cortex.The BDNF immunopositive reaction was seen in the plasma and neurite of neurons in the layer III and V cerebral cortex of monkey.Conclusions Electrophoresis can isolate many proteins from the cerebral cortex of Rhesus monkey.BDNF can be recognized and indentified by Western blot.BDNF is localized in the neurons of the cerebral cortex of monkey,suggestsing that BDNF may be a crucial molecule in the functional maintainence of CNS in adult monkeys.

关 键 词：恒河猴 大脑皮质 WESTERN BLOT BDNF 组织分布 

分 类 号：R651[医药卫生—外科学]