三七总皂苷对肺心病兔肺纤维化组织Cathepsin B表达的影响  

Pathological Study of PNS in Regulating Cathepsin B Expression in Rabbits with Pulmonary Fibrosis of Pulmonary Heart Disease

作　　者：张济周[1] 李青[2] 林萍[3] 杨梁梓[2] 宋精玲[3] 

机构地区：[1]温州市中医院肿瘤科,浙江温州325000 [2]昆明医学院第二附属医院中西医结合科,云南昆明650101 [3]昆明医学院病理教研室,云南昆明650031

出　　处：《昆明医学院学报》2011年第1期36-40,54,共6页Journal of Kunming Medical College

基　　金：云南省卫生厅科研基金资助项目(2009NS065)

摘　　要：目的从病理学角度研究三七总皂苷对肺心病兔肺纤维化Cathepsin B表达的调控.方法健康日本大耳兔40只,随机分为五组,每组8只,分别为正常对照组、肺心病模型组、三七总皂苷预防组、治疗组及西药治疗组.观察光镜下五组动物CB蛋白的表达及肺组织的病理结构变化.结果与模型组相比,三七总皂苷预防组能明显减轻肺泡水肿,减轻肺间质、肺细小动脉及肺泡壁纤维化,明显减少肺泡水肿液及炎细胞渗出,降低CB蛋白在肺组织中的表达;三七总皂苷治疗组及西药治疗组也有上述作用,但不及三七总皂苷预防组明显.结论三七总皂苷能明显改善肺心病兔肺纤维化的程度,显降低CB蛋白在支气管上皮细胞和肺泡上皮细胞的表达,减轻致纤维化因素对肺间质、肺部细小动脉及肺泡壁的病理损伤,对肺心病肺脏损伤具有保护作用.Objective To investigate the effect of PNS in regulating the expression of Cathepsin B in rabbits with pulmonary fibrosis of pulmonary heart disease from the perspective of pathology.Methods Forty healthy Japanese rabbits were randomly divided into five groups（eight rabbits in each group）：the control group,the PF model group,the PNS prevention group,treatment group and the western medicine treatment group.The expression of CB protein and the pathological structural changes in lung tissue were observed under light microscope.Results Compared with the model group,PNS significantly reduced the pulmonary edema,reducing pulmonary interstitial,pulmonary arteries and alveolar wall fibrosis,significantly reduced pulmonary edema fluid and inflammatory cell exudate,reducing CB protein in lung tissue expression of rabbits in the prevention group.PNS treatment group and western medicine treatment group also had effects,but the effects were significantly less than the PNS prevention group.Conclusions PNS can significantly improve the extent of pulmonary fibrosis,reduce the CB protein levels in bronchial epithelial cells,alveolar epithelial cells,reduce the inducing factors of pulmonary interstitial fibrosis,pulmonary arterioles and alveolar wall＇s pathological damage,in rabbits with pulmonary heart disease.Therefore,PNS can protect lung of rabbits with pulmonary heart disease against damage.

关 键 词：三七总皂苷 肺心病 肺纤维化 兔 CATHEPSIN B 表达调控 病理学 

分 类 号：R285.5[医药卫生—中药学]