不同剂量白藜芦醇对星形胶质细胞Aβ损伤的影响  

Effects of resveratrol on β-amyloid-induced injury in astrocytes

作　　者：孔丹莉[1,2] 郭江[1] 陈清[1] 周浩[3] 高璐璐[1] 黎文明[1] 俞守义[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院,广东广州510515 [2]广东医学院公共卫生学院,广东湛江524023 [3]南方医科大学南方医院感染管理科,广东广州510515

出　　处：《热带医学杂志》2011年第1期11-14,F0004,共5页Journal of Tropical Medicine

摘　　要：目的研究白藜芦醇对β淀粉样蛋白诱导星形胶质细胞的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)及丙二醛(MDA)释放的影响和其对氧化损伤的保护作用,从而探索其对中枢神经保护作用的可能机制。方法采用不同浓度的白藜芦醇预处理星形胶质细胞12h,继之用25μmol/L浓度的β淀粉样蛋白进行损伤,然后检测细胞SOD、GSH-PX及MDA释放的含量,并对数据进行统计学分析。结果β淀粉样蛋白损伤后,细胞SOD、GSH-PX的释放量明显下降(P<0.05),MDA的释放量升高(P<0.05),而用1、10、100μmol/L白藜芦醇预处理则能升高损伤细胞的GSH-PX的释放量(P<0.05),并能降低损伤细胞MDA的释放量(P<0.05);10、100μmol/L白藜芦醇预处理则能升高损伤细胞的SOD的释放量(P<0.05)。结论星形胶质细胞受到β淀粉样蛋白损伤后,白藜芦醇能增加SOD和GSH-PX的释放量,降低MDA的释放量,提示白藜芦醇对星形胶质细胞具有保护作用。Objective To study the effects of resveratrol on β-amyloid-induced superoxide dismutase（SOD）,glutathione peroxide（GSH-PX） and MDA in cultured astrocytes and the protective effects of resveratrol on the central nervous system.Methods Astrocytes were treated with various concentrations of resveratrol for 12 h and then exposed to Aβ.Cell viability was measured by CCK-8 assay.The levels of SOD,GSH-PX and MDA were examined after injury.Results After treatment with resveratrol（1,10 and 100 μmol/L）,the MDA intensity was decreased（P0.05）.At the concentrations of 10 and 100 μmol/L,resveratrol elevated the levels of SOD and GSH-PX in Aβ injuried astrocytes（P0.05）.Conclusion Resveratrol may have cytoprotective effect through the increase of SOD and GSH-PX,and the decrease of MDA.

关 键 词：Β淀粉样蛋白 阿尔茨海默病 白藜芦醇 星形胶质细胞 氧化损伤 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]