Preliminary molecular epidemiology of the Staphylococcus aureus in lower respiratory tract infections： a multicenter study in China  

作　　者：LI De-zhi CHEN Yu-sheng YANG Jing-ping ZHANG Wei HU Cheng-ping LI Jia-shu MU Lan HU Ying-hui GENG Rong HU Ke CAI Shao-xi WAN Huan-ying WANG Qiu-yue WEI Li-ping DU Juan YU Qin ZHONG Xiao-ning WANG Rui-qin MA Jian-jun TIAN Gui-zhen WANG Si-qin GAO Zhan-cheng 

机构地区：[1]Department of Respiratory and Critical Care Medicine, Peking University People's Hospital, Beijing 100044, China [2]Department of Respiratory Medicine, Fujian Provincial Hospital, Fuzhou, Fujian 350001, China [3]Department of Respiratory Medicine, Third Affiliated Hospital of the Inner Mongolia Medical College, Baotou, Inner Mongolia 014010, China [4]Department of Respiratory Medicine, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China [5]Department of Respiratory Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China [6]Department of Respiratory Medicine, Lianyungang First People's Hospital affiliated of Xuzhou Medical College, Lianyungang, Jiangsu 222002, China [7]Department of Respiratory Medicine, Peking University Space Central Hospital, Beijing 100049, China [8]Department of Respiratory Medicine Beijing Children's Hospital affiliated of Capital Medical University, Beijing 100045, China [9]Department of Emergency Medicine , Beijing Children's Hospital affiliated of Capital Medical University, Beijing 100045, China [10]Department of Respiratory Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China [11]Department of Respiratory Medicine, Nanfang Hospital affiliated to Southern Medical University, Guangzhou, Guangdong 510515, China [12]Department of Respiratory Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University, Shanghai 200025, China [13]Institute of Respiratory Disease, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China [14]Department of Respiratory Medicine, Third Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong 510150, China [15]Department of Respiratory Medicine, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou 550004, China [16]Department of Respiratory Medicine, First Hospital of Lanzhou University, Lanzhou, Gansu 730000, China [17]Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanni

出　　处：《Chinese Medical Journal》2011年第5期687-692,共6页中华医学杂志（英文版）

摘　　要：Background Staphylococcus aureus （S. aureus） remains as an important microbial pathogen resulting in community and nosocomial acquired infections with significant morbidity and mortality. Few reports for S. aureus in lower respiratory tract infections （LRTIs） have been documented. The aim of this study was to explore the molecular epidemiology of S. aureus in LRTIs in China.Methods A multicenter study of the molecular epidemiology of S. aureus in LRTIs was conducted in 21 hospitals in Beijing, Shanghai and twelve other provinces from November 2007 to February 2009. All the collected S. aureus strains were classified as minimum inhibitory concentration （MIC）, mecA gene, virulence genes Panton-Valentine Leukocidin （PVL） and y-hemolysin （hlg）, staphylococcal cassette chromosome mec （SCCmec） type, agr type, and Multilocus Sequence Typinq （MLST）.Results Totally, nine methicillin-sensitive S. aureus （MSSA） and 29 methicillin-resistant S. aureus （MRSA） strains were isolated after culture from a total of 2829 sputums or bronchoalveolar lavages. The majority of MRSA strains （22/29） had a MIC value of 〉512 μg/ml for cefoxitin. The mecA gene acting as the conservative gene was carried by all MRSA strains. PVL genes were detected in only one S. aureus strain （2.63%, 1/38）. The hlg gene was detected in almost the all S. aureus （100% in MSSA and 96.56% in MRSA strains）. About 75.86% of MRSA strains carried SCCmec Ⅲ. Agr type 1 was predominant （78.95%） among the identified three agr types （agr types 1,2, and 3）. Totally, ten sequence type （ST） of S. aureus strains were detected. A new sequence type （ST1445） was found besides confirming ST239 as the major sequence type （60.53%）. A dendrogram generated from our own MLST database showed all the bootstrap values 〈50%. Conclusion Our preliminary epidemiology data show SCCmec Ⅲ, ST239 and agr type 1 of S. aureus as the predominant strains in LRTIs in Mainland of China.Background Staphylococcus aureus （S. aureus） remains as an important microbial pathogen resulting in community and nosocomial acquired infections with significant morbidity and mortality. Few reports for S. aureus in lower respiratory tract infections （LRTIs） have been documented. The aim of this study was to explore the molecular epidemiology of S. aureus in LRTIs in China.Methods A multicenter study of the molecular epidemiology of S. aureus in LRTIs was conducted in 21 hospitals in Beijing, Shanghai and twelve other provinces from November 2007 to February 2009. All the collected S. aureus strains were classified as minimum inhibitory concentration （MIC）, mecA gene, virulence genes Panton-Valentine Leukocidin （PVL） and y-hemolysin （hlg）, staphylococcal cassette chromosome mec （SCCmec） type, agr type, and Multilocus Sequence Typinq （MLST）.Results Totally, nine methicillin-sensitive S. aureus （MSSA） and 29 methicillin-resistant S. aureus （MRSA） strains were isolated after culture from a total of 2829 sputums or bronchoalveolar lavages. The majority of MRSA strains （22/29） had a MIC value of 〉512 μg/ml for cefoxitin. The mecA gene acting as the conservative gene was carried by all MRSA strains. PVL genes were detected in only one S. aureus strain （2.63%, 1/38）. The hlg gene was detected in almost the all S. aureus （100% in MSSA and 96.56% in MRSA strains）. About 75.86% of MRSA strains carried SCCmec Ⅲ. Agr type 1 was predominant （78.95%） among the identified three agr types （agr types 1,2, and 3）. Totally, ten sequence type （ST） of S. aureus strains were detected. A new sequence type （ST1445） was found besides confirming ST239 as the major sequence type （60.53%）. A dendrogram generated from our own MLST database showed all the bootstrap values 〈50%. Conclusion Our preliminary epidemiology data show SCCmec Ⅲ, ST239 and agr type 1 of S. aureus as the predominant strains in LRTIs in Mainland of China.

关 键 词：Staphylococcus aureus lower respirato tract infections molecular epidemiology staphylococcal cassette chromosome mec Multilocus Sequence Typing 

分 类 号：S852.659.5[农业科学—基础兽医学] TS207.4[农业科学—兽医学]