Granulocyte-macrophage colony-stimulating factor and interleukin 4 induce the malignant transformation of the bone marrow- derived human adult mesenchymal stem cells  

作　　者：ZHOU Xiao-gang YANG Yi YANG Jin-song ZHOU Jian FANG Tao-lin DAI Wen-da CHEN Zheng-rong 

机构地区：[1]Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China [2]Department of Orthopedics, The First Affiliated Hospital of Gaungxi Medical University, Nanning, Guangxi 530021, China

出　　处：《Chinese Medical Journal》2011年第5期729-733,共5页中华医学杂志（英文版）

摘　　要：Background The purpose of the study was to examine the effects of granulocyte-macrophage colony-stimulating factor （GM-CSF） and interleukin 4 （IL-4） on the bone-marrow-derived human adult mesenchymal stem cells （hMSCs）. Methods The hMSCs were isolated and cultured with GM-CSF and IL-4 for a period of one month. A single colony of transformed cells was then isoloated and their phenotype was characterized by morphology, surface marker expression, and in vivo tumorigenesis.Results After one month culture, the transformed mesenchymal cells exhibited the morphology and phenotype similar to those of tumor cells, and also caused multiple fast growing lung deposits when it was injected into immunodeficient mice.Conclusion Cytokines-driven malignant transformation of hMSCs may be a useful model for studying signaling pathways initiating malignant transformation of hMSC.Background The purpose of the study was to examine the effects of granulocyte-macrophage colony-stimulating factor （GM-CSF） and interleukin 4 （IL-4） on the bone-marrow-derived human adult mesenchymal stem cells （hMSCs）. Methods The hMSCs were isolated and cultured with GM-CSF and IL-4 for a period of one month. A single colony of transformed cells was then isoloated and their phenotype was characterized by morphology, surface marker expression, and in vivo tumorigenesis.Results After one month culture, the transformed mesenchymal cells exhibited the morphology and phenotype similar to those of tumor cells, and also caused multiple fast growing lung deposits when it was injected into immunodeficient mice.Conclusion Cytokines-driven malignant transformation of hMSCs may be a useful model for studying signaling pathways initiating malignant transformation of hMSC.

关 键 词：bone marrow-derived mesenchymal stem cells malignant transformation interleukin 4 granulocyte-macrophage colony-stimulating factor 

分 类 号：O[理学]