新型近红外试剂的合成及其现场二聚体与DNA作用的研究  

Synthesis of a Novel Near IR Fluorescent Reagent and Study on its in situ Dimers Interaction with DNA

作　　者：郑洪[1] 李东辉[1] 吴敏 陈秋影[1] 许金钩[1] 

机构地区：[1]厦门大学化学系

出　　处：《高等学校化学学报》1999年第7期1026-1030,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：合成了一种新型近红外阴离子染料，并对其水溶液及阳离子表面活性剂ＣＴＡＢ存在下的吸收及荧光光谱进行了研究．结果表明，低浓度的ＣＴＡＢ与该近红外阴离子染料形成离子缔合物而使阴离子染料的荧光强度降低，当ＣＴＡＢ的浓度进一步加大时，其胶束前预聚集促使该染料形成非荧光二聚体，导致荧光急剧猝灭．当ＣＴＡＢ浓度大于临界浓度时，染料二聚体解离，染料单体增溶于胶团中形成高量子产率荧光体且最大发射波长从７９５ｎｍ红移至８１２ｎｍ．对该近红外阴离子染料在ＣＴＡＢ的分子胶束前预聚集过程中现场形成的弱荧光二聚体作为一种双螺旋ＤＮＡ的荧光测定探针的可行性做了初步的研究。A new anionic Near IR fluorescent cyanine dye was synthesized and its absorption and fluorescence spectra in water and in the solution of cationic surfacant hexadecyltrimethyl ammonium bromide(CTAB) were studied. The results showed that the maximum excitation wavelength and emission wavelength were 765 nm and 795 nm in water, respectively. In the presence of low concentration of CTAB, the dye formed ionic associate with CTAB was made higher to form the premiceller aggregation, the fluorescence intensity decreased dramatically because of forming a low fluorescent in situ dimeric dye. When the concentration of CTAB was above the critical micelle concentration(cmc), the dimeric dye dissociated, and the fluorescence intensity of Near IR cyanine was restored, and the maximum emission wavelength was bathochromated from 795 nm to 812 nm. The possibility of using low fluorescent in situ dimeric dye as a Near IR region fluorescent probe for DNA was also studied.

关 键 词：近红外荧光检测 试剂 染料 CTAB DNA 荧光光谱 

分 类 号：TQ421.383[化学工程] Q523.03[生物学—生物化学]