慢性饮酒对大鼠胃缺血再灌注损伤影响  

Influence of chronic alcohol intake on I-R induced gastric mucosa injury in rats

作　　者：马小波[1] 王洪建[1] 徐庆雷[1] 杜东书[2] 

机构地区：[1]沭阳县人民医院检验科,江苏沭阳223600 [2]徐州医学院

出　　处：《中国公共卫生》2011年第4期453-454,共2页Chinese Journal of Public Health

摘　　要：目的探讨酒精对大鼠胃缺血再灌注损伤的影响机制。方法制备胃缺血再灌注及慢性饮酒大鼠模型,实验分为6组:假手术、饮酒假手术、胃缺血再灌注1、2 h组、饮酒胃缺血再灌注1、2 h组,每组6只;记录各组胃黏膜损伤指数并检测胃黏膜丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性;缺口末端标记法(TUNEL)检测胃黏膜细胞凋亡情况。结果缺血再灌注1、2 h后胃黏膜损伤指数分别为(106.0±12.0)和(74.4±11.0),黏膜细胞凋亡率分别为(73.2±6.5)%和(54.0±7.4)%,明显高于假手术组(P<0.05);与假手术组比较,缺血再灌注1 h后胃黏膜MDA含量[(7.98±2.1)nmol/mgprot]明显增加,SOD活性[(141±16.1)U/mgprot]明显降低;饮酒可引起胃黏膜轻微损伤(损伤指数为9.2±0.7),但饮酒并未加剧缺血再灌注带来的胃黏膜损伤,仅使机体自身修复能力降低。结论饮酒可导致SOD活性下降,加剧机体内氧化和抗氧化平衡失调,使胃黏膜自身修复损伤能力降低。Objective To investigate the effect of alcohol on ischemia-reperfusion（I-R） induced gastric mucosa injury in rats. Methods The rat model of gastric I-R injury and chronic alcohol drinking was established and the rats were randomly divided into 6 groups：normal sham operation group,alcohol drinking sham operation group,normal gastric I-R 1hr/2hr groups,and alcohol drinking gastric I-R 1hr/2hr groups.Gastric mucosal injury index was recorded.The contents of malondialdehyde（MDA） and the activity of superoxidase dismutase（SOD） in gastric mucosa were measured.The status of gastric mucosa cellula apoptosis was detected by TdT-mediated dUTP-biotin nick end labeling（TUNEL）. Results I-R induced gastric mucosa injury（P0.05）.The gastric mucosa injury index was 106 ±12 and 74.4 ±11 after the reperfusion of 1 hr and 2 hr with the mucosal apoptosis rate of 73.2±6.5% and 54±7.4%,respectively.Compared with that of normal sham group,the content of MDA（7.98±2.1 nmol/mgprot） was significantly increased and the activity of SOD（141±16.1 U/mgprot） was reduced after gastric I-R of 1 hour.Gastric mucosal injury index（9.2±0.7） was increased after chronic alcohol intake.Chronic alcohol drinking didn＇t aggrevated I-R injury,but weakened the ability of gastric self-repair. Conclusion Alcohol intake could reduce the activity of SOD and aggravate the oxidation and antioxidant balance disorders,and then weaken the ability of gastric self-repair.

关 键 词：胃缺血再灌注 乙醇 胃黏膜损伤指数 胃黏膜细胞凋亡 

分 类 号：R333.2[医药卫生—人体生理学]