食品中正二氢愈疮酸含量的高效液相色谱荧光法测定  

Simple and Rapid Analysis of Nordihydroguaiaretic Acid Contained in Food by HPLC Fluorescence Detection

作　　者：段沅杏[1,2] 贺兵[2] 李干鹏[1] 张承明[2] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031 [2]云南省烟草化学重点实验室云南烟草科学研究院,云南昆明650106

出　　处：《云南化工》2011年第1期42-47,共6页Yunnan Chemical Technology

摘　　要：建立了高效液相色谱荧光法(HPLC-FLD)测定食品中正二氢愈疮酸含量的方法。采用Luna C18(4.6×150 nm,3.0μm)的色谱柱,以0.1%甲酸乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,流速1.0 mL/min;激发波长为280nm,发射波长为306nm。在优化的前处理和色谱条件下,正二氢愈疮酸能够与样品基质完全分离,且加标回收率范围为92.43%～99.23%。Simple and rapid analysis of nordihydroguaiaretic acid（NDGA） in food by HPLC-FLD was developed.The important factors affecting separation and detection were investigated in detail.Extraction of NDGA from samples was accomplished by 90% methanol with 0.1% ascorbic acid.Furthermore,nordihydroguaiaretic acid was separated on a Luna stable bound（4.6×150nm,3.0μm） C18 column with mixture of acetonitrile-water-formic acid（v/v） as the mobile phase and fluorescence detector was effective in isolating NDGA from most of food additives.The standard recoveries were ranged from 93.74%~99.61%.Our results suggest that this is a simply,quickly and sensitively analytical method to detect NDGA in food.

关 键 词：正二氢愈疮酸 高效液相色谱法 荧光检测器 

分 类 号：O657.72[理学—分析化学]