富血小板血浆对血管内皮细胞增殖及血管形成的影响  

Effects of platelet-rich plasma on the proliferation and vasoformation of human umbilical vascular endothelial cells in vitro

作　　者：张云松[1] 何井华[3] 戴丽冰[2] 黎庆梅[2] 

机构地区：[1]暨南大学第四附属医院,广州市红十字会医院整形科,510220 [2]暨南大学第四附属医院,广州市红十字会医院医学实验中心,510220 [3]南方医科大学附属珠江医院全科医学科,广州市510282

出　　处：《实用医学杂志》2011年第7期1145-1147,共3页The Journal of Practical Medicine

基　　金：广东省自然科学基金(编号:8451022001001839);广东省医学科学技术研究基金(编号:A2009525);广州市医药卫生科技项目(编号:2008-YB-040)

摘　　要：目的:探讨富血小板血浆(platelet-rich plasma,PRP)对人脐静脉血管内皮细胞(human umbilical vein vessel endothelial cells,HUVECs)增殖及体外形成血管的影响。方法:体外培养HUVECs细胞系,二次离心法制备自体富血小板血浆,将细胞分为实验组与对照组,实验组以含5%、10%、20%自体富血小板血浆的条件培养液干预,对照组不进行干预。采用四甲基偶氮唑蓝(XTT)法测定HUVECs的增殖情况,流式细胞仪检测细胞周期,体外成管实验检测细胞形成血管的能力。结果:富血小板血浆实验组较对照组细胞形态饱满、密度增大;XTT比色法显示各浓度实验组细胞增殖与对照组比较明显增高(P<0.01);流式细胞仪检测实验组S期细胞明显增多(P<0.01);体外成管实验显示实验组细胞能形成完整的管状结构,小管面积明显大于对照组(P<0.01),对照组小管形成结构不完整、面积小于实验组(P<0.05)。结论:PRP能在体外明显促进HUVECs的增殖及形成毛细血管腔,在组织再生或修复过程中可能发挥促新生血管形成的作用。Objective To investigate the effects of platelet-rich plasma （PRP） on the proliferative and capillary canaliculization of human umbilical vascular endothelial cells in vitro. Methods PRP was prepared by twice centrifugal method, and HUVECs （CEV-304） were cultured in vitro and then randomly divided into experimental group and control group. Special culturing media including 5%, 10%, and 20% PRP were added into the HUVECs separately in the experimental group and none in the control group. The proliferation of HUVECs was measured by XTT assay, the cell cycle was tested by flow cytometry, and the ability of capillary canaliculization of HUVECs was tested by tube formation assay. Results HUVECs in experimental group had better shape and higher density than those in control group. XTT method showed that the proliferative activity of HUVECs in experimental group was obviously stronger than that in control group （P 〈0.01 ）. As compared to the cells in control group, those in S-phase were increased in experimental group （P 〈0.01 ）, and formed a complete canaliculization and the area of canaliculus was significantly bigger （P 〈 0.05）. Conculusions PRP can promote the proliferation and the capillary canaliculization of HUVECs in vitro, and may promote the angiogenesis in the process of tissue regeneration.

关 键 词：富血小板血浆 血管内皮细胞 细胞增殖 血管形成 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]