不同品系大鼠皮下脂肪源干细胞诱导分化能力的比较  

The Comparison of differentiation potency of adipose-derived stem cells from sprague-dawley rats and wistar rats

作　　者：曲戎梅[1] 姚红卫[2] 戴景兴[3] 陈白虹[1] 张艳玲[1] 林来兴妹[1] 原林[3] 

机构地区：[1]南方医科大学生物技术学院教学科研实验中心,广州市510515 [2]南方医科大学南方医院药局,广州市510515 [3]南方医科大学基础医学院人体解剖教研室,广州市510515

出　　处：《实用医学杂志》2011年第7期1151-1153,共3页The Journal of Practical Medicine

基　　金：国家重点基础研究计划(973计划)项目(编号:2007CB512705);国家自然基金青年面上项目(编号:30801464)

摘　　要：目的:对比SD大鼠和Wistar大鼠皮下脂肪源干细胞(adipose-derived stem cells,ADSCs)体外诱导分化能力,为ADSCs的进一步应用提供实验依据。方法:SD大鼠和Wistar大鼠各6只取腹股沟脂肪垫,培养扩增ADSCs,相差显微镜下观察两个品系来源的ADSCs的形态。用第4代细胞进行成骨和成脂诱导,分别用茜素红和油红O染色观察向成骨细胞分化后的矿化结节和向脂肪细胞分化后的脂质沉积。结果:两个品系来源的ADSCs在细胞形态上没有显著区别,都能进行成骨和成脂诱导分化。结论:SD大鼠和Wistar大鼠腹股沟脂肪垫来源的ADSCs诱导分化能力差异无显著性。Objective To investigate the difference of differentiation potency of adipose-derived stem cells （ADSCs） from Spragne-Dawley （SD） rats and Wistar Rats. Methods ADSCs were isolated from the fat pad located in pars inguinalis of 6 SD rats and 6 Wistar rats and cultured in vitro. The morphology of ADSCs was observed using phase-contrast microscopy. ADSCs at generation 4th were cultured under adipogenic and osteogenic condition to conform their differentiation potential. The morphological characteristics of inductive cells were observed using histological staining such as alizarin red for mineralization nodules and oil red 0 for lipid accumulation during osteogenic and adipogenic induction. Results There was no significant difference in cell morphology of ADSCs between SD rats and Wistar rats. Both of them have the capacity to differentiate toward the adipogenic and osteogenic lineages. Conclusion ADSCs obtained from SD rats show similar cell differentiation potential to those obtained from Wistar rats. Therefore, these two kinds of ADSCs are both favorable choice for stem cell transplantation and tissue engineering.

关 键 词：干细胞 分化 腹股沟脂肪垫 成脂肪诱导 成骨诱导 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]