用放射性配体结合分析法测定乳癌细胞泌乳素受体  

Receptor Radioassay of Prolactin Receptors in Breast Cancer Cells

作　　者：刘孟元[1,2] 周肃[1,2] 汤特[1,2] 

机构地区：[1]天津医学院病理生理教研室 [2]天津市内分泌研究所促激素室

出　　处：《天津医药》1990年第7期393-396,共4页Tianjin Medical Journal

摘　　要：本研究以~125I-oPRL为放射性标记配体,对乳癌细胞泌乳素受体进行放射分析检测。证实乳癌细胞存在泌乳素特异性受体,该受体对泌乳素结合具有高度亲和力(Kd=1.19×10^(-10)M Bmax=9.39fmol/mg)结合反应受孵育时间和温度的影响,而且是可以饱和的。将受体膜用高浓度(4M)MgCl_2处理,可提高可测受体的数目,增强检测方法的敏感性。乳癌细胞泌乳素受体的放射分析对乳癌的基础研究和指导乳癌的治疗、估计预后均有重要意义。Crude membrane fractions prepared from human breast cancer by homogenizationand differential centrifugation were incubated with labeled ovine prolactin in thepresence or absence of increasing amounts of hormones.Scatchard analysis of thebinding of ^(125)Ⅰ-labeled ovine prolactin to receptors indicated that human breast cancercell contained a specific receptor for prolactin inn low quantity(Bmax=9.39 fmol/mgprotein)and high affinity(Kd=l.19×10^(-10)M).When these receptors were desaturatedfrom endogenous prolactin by exposure to 4M MgCl_2,the number of available reoeptorsin crude membrane fractions was increased more than two-fold.

关 键 词：乳癌 放射性配体 乳腺肿瘤 

分 类 号：R817.1[医药卫生—影像医学与核医学]