对氨基水杨酸钠干预锰致大鼠海马神经元损伤的体外研究  

Intervention Effects of Sodium P-amino Salicylate on Manganese Induced Damage of Rat Hippocampal Neurons in Vitro

作　　者：王芳[1] 王禅[1] 姜岳明[1] 邓祥发[2] 陆继培[1] 区仕燕[1] 

机构地区：[1]广西医科大学公共卫生学院,广西南宁530021 [2]广西医科大学基础医学院,广西南宁530021

出　　处：《工业卫生与职业病》2011年第2期82-85,共4页Industrial Health and Occupational Diseases

基　　金：国家自然科学基金项目(30760210;81072320);广西教育厅研究生教育创新计划(2008105981004D32)

摘　　要：目的探讨对氨基水杨酸钠(PAS-Na)对体外染锰致大鼠原代海马神经元损伤的干预作用。方法培养至第8天的海马神经元随机分为对照组、染锰组、低、中和高剂量PAS-Na干预组(L-PAS、M-PAS、H-PAS组)。对照组给予培养液培养48 h;染锰组神经元暴露于MnCl2·4H2O 50μmol/L培养液培养24 h后;L-PAS、M-PAS、H-PAS组神经元暴露于MnCl2·4H2O 50μmol/L培养液培养24 h后,分别给予含PAS-Na 50、500、5000μmol/L的培养液作用24 h。然后,用噻唑蓝(MTT)法测定神经元存活率,Annexin V-FITC细胞凋亡试剂盒检测神经元凋亡率和Rhodarnine123检测神经元线粒体平均荧光强度。结果染锰组神经元存活率明显低于对照组,神经元凋亡率和线粒体平均荧光强度高于对照组,差异有统计学意义(P<0.05)。L-PAS、M-PAS、H-PAS干预组与染锰组上述指标比较,差异无统计学意义(P>0.05)。结论体外染锰对大鼠原代海马神经元损伤明显,但未观察到PAS-Na对锰海马神经元毒性有干预作用。Objective To explore intervention effect of sodium p-amino salicylate（PAS-Na） on damaged primary cultured neurons in hippocampus of manganese（Mn） exposed rat.Methods Neurons in hippocampus of rat cultured for 8 days were randomly divided into the control group,Mn exposed group,low,medium and high doses PAS-Na（L-PAS,M-PAS and H-PAS） intervention groups.The control neurons were cultured for 48 h only with incubate medium.Mn exposed neurons were firstly exposed to culture medium plus Mn（MnCl2／54H2O 50 μmol/L） for 24 h and then further cultured for 24 h.Neurons of PAS intervention groups were firstly exposed to medium plus Mn（MnCl2／54H2O 50 μmol/L） for 24 h,and then cultured with mediums plus PAS-Na（50,500 and 5000 μmol/L） as the L-PAS,M-PAS and H-PAS groups,respectively for another 24h.Cell viability,apoptosis ratio and mean of fluorescence intensity in mitochondria were determined by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）,annexin V-FITC apoptosis assay and rhodarnine123.Results Viability of cells in Mn exposed group was significantly lower than that of the control group（P0.05）.Apoptosis rate in neuron and mean of fluorescence intensity in mitochondria of Mn exposed group were higher than those of the control group（P0.05）.However,no significant changes of cell viability,apoptosis ratio and mean of fluorescence intensity in mitochondria in L-PAS,M-PAS and H-PAS groups were found in comparison with Mn exposed group（P0.05）.Conclusions This study demonstrates that manganese causes obvious damage of primary cultured neurons in hippocampus of rat.However,the intervention effect of PAS-Na on the hippocampal toxicity induced by manganese is not observed.

关 键 词：锰 对氨基水杨酸钠 大鼠 海马神经元 体外研究 

分 类 号：R135.1[医药卫生—劳动卫生]