香蕉棒孢霉叶斑病菌的分离与鉴定  

Isolation and Identification of Corynespora cassiicola Causing Banana Leaf Spot Disease

作　　者：林善海[1] 黄思良[2] 张贺[3] 漆艳香[3] 覃丽萍[4] 谢玲[4] 付岗[1,4] 

机构地区：[1]广西大学农学院,广西南宁530004 [2]南阳师范学院生命科学与技术学院,河南南阳473061 [3]中国热带农业科学院环境与植物保护研究所,海南儋州571737 [4]广西农业科学院微生物研究所,广西南宁530007

出　　处：《热带作物学报》2011年第1期152-157,共6页Chinese Journal of Tropical Crops

基　　金：国家香蕉产业技术体系(nycytx-33);广西科学基金(桂科自0728071)

摘　　要：从广西南宁市采集香蕉棒孢霉叶斑病样本,用常规组织分离法获得3个菌株,经柯赫氏法则验证确定其为香蕉叶斑病的病原菌。该菌的形态特征在分离培养前后变化较大,自然病斑上的分生孢子和分生孢子梗均较短,经PDA培养基分离培养后显著变长,培养后的病原菌形态特征与前人对Corynespora cassiicola的描述一致。根据形态特征及rDNA-ITS区序列比对结果,确定该病原菌株为多主棒孢菌[Corynespora cassiicola(Berk&Curt)Wei]。Three isolates were obtained from banana leaf spot samples collected from Nanning,Guangxi by tissue isolation.The pathogenicity of the isolates was confirmed based on Koch’s postulates.The conidia and conidiophores produced on the PDA-incubated colonies became longer than those on naturally occurred banana leaf spots.The pathogenic isolate CATAS100 was identified as a member of Corynespora cassiicola based on its morphological characters on PDA medium and rDNA-ITS（ribosomal DNA internal transcribed spacer） sequence.This is the first report on molecular identification of Corynespora cassiicola causing banana leaf spots in China.

关 键 词：多主棒孢菌 症状 分生孢子 分生孢子梗 rDNA—ITS 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]