锂剂对SH-SY5Y细胞氧糖剥夺模型的保护作用及其对AIF凋亡通路的影响  

The protective effect of lithium on the oxygen and glucose deprivation model of SH-SY5Y cells and its influence on AIF apoptosis pathway

作　　者：贾国强[1] 巴晓红[1] 潘凤英[1] 

机构地区：[1]辽宁医学院附属第一医院神经内科,辽宁锦州121000

出　　处：《中风与神经疾病杂志》2011年第3期228-232,共5页Journal of Apoplexy and Nervous Diseases

摘　　要：目的研究不同浓度的锂剂对SH-SY5Y细胞氧糖剥夺模型的保护效果,确定有效的浓度,并探讨其对AIF凋亡通路的作用,分析其神经保护作用的机制。方法以不同浓度的锂剂对制备氧糖剥夺模型的SH-SY5Y细胞进行预处理,观察其对细胞的保护作用。MTT法描绘细胞生长曲线。Annexin V和PI双染,流式细胞仪检测各组细胞凋亡情况。免疫荧光检测凋亡诱导因子(AIF)蛋白的变化。结果 MTT显示不同浓度的锂剂对SH-SY5Y细胞氧糖剥夺模型有较强的细胞保护作用,1mmol/L氯化锂为细胞保护的有效剂量。Annexin V和PI双染,流式细胞仪检测可见锂剂处理组凋亡细胞明显减少。免疫荧光染色可见锂剂处理组AIF蛋白发生核移位减少。结论锂剂对氧糖剥夺模型的SH-SY5Y细胞具有较强的保护作用,1mmol/L氯化锂为细胞保护的有效剂量,其作用机制之一可能是对AIF凋亡通路的抑制。Objective To study the protective effects of the different concentrations of lithium on the SH-SYSY cells oxygen and glucose deprivation model, then to determine the effective concentration, and to explore its influence on AIF apoptosis pathway and the mechanism of its neuroprotective effect. Methods Different concentrations of lithium preconditioned oxygen and glucose deprivation model of SH-SY5Y cells were used. MTT staining were used to describe the effects of the drugs. The cell apoptotic rate was analyzed by flow cytometry after Annexin V/PI double staining. AIF protein was detected by immunofluorescence staining. Results MTF showed that different concentrations of lithium had strong protective effects on the oxygen and glucose deprivation model of SH-SYSY cells, and 1 mmol/L lithium chloride was the effective dose for cell protection. After Annexin V and PI staining, flow cytometry showed that the apoptosis was decreased significantly in cells treated with lithium. Immunofiuorescence staining suggested that AIF protein reduced in nuclear area after treated with lithium. Conclusion Lithium has strong protective effect on the oxygen and glucose deprivation model of SH-SYSY cells, l mmol/L lithium chloride is the effective dose for cell protection, and one of the mechanisms may be inhibition of AIF apoptosis pathway.

关 键 词：SH—SY5Y细胞 AIF凋亡通路 锂剂 氧糖剥夺模型 

分 类 号：R453[医药卫生—治疗学]