养血生发胶囊对小鼠肝毒性“量-时-毒”关系的病理学研究  

Pathologic Research on Hepatotoxicity in Mice Caused by Yangxue Shengfa Capsules

作　　者：吕莉莉[1] 谢元璋[1,2] 钱晓路[3] 杨倩[3] 孙蓉[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]哈尔滨商业大学,黑龙江哈尔滨150000 [3]山东中医药大学,山东济南250355

出　　处：《中国药物警戒》2011年第4期202-204,共3页Chinese Journal of Pharmacovigilance

基　　金：国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802);山东省科技平台建设项目课题(2008GG2NS02021)

摘　　要：目的研究养血生发胶囊单次给药对小鼠造成的肝毒性在病理学上的损伤程度,为养血生发胶囊肝毒性损伤提供病理组织学依据。方法采用常规HE染色方法,通过光镜下阅片观察和病理图像分析,观察养血生发胶囊单次灌胃对小鼠肝毒性＂量-时-毒＂关系研究中的病理学变化。结果 ＂时-毒＂关系研究：给药后2h检测发现,在给药后6～24h表现较明显。＂量-毒＂关系研究：在2.50~12.00g.kg-1之间出现不同程度的病理组织学损伤,本品导致的主要病理改变为：肝细胞嗜酸性变、玻璃样变、脂肪变、空泡变等。结论单次给小鼠灌胃不同剂量的养血生发胶囊可造成急性肝损伤,并呈现一定的＂量-时-毒＂关系,并可导致肝细胞出现嗜酸性变、玻璃样变、脂肪变、空泡变等病理学改变。Objective To investigate the pathologic injury degree of hepatotoxicity in mice induced by single administration of Yangxue Shengfa Capsules and provide pathohistological basis for its hepatotoxical injury.Methods The pathologic changes of mice in the study on ＂dose-time-toxicity＂relationship of hepatotoxicity caused by Yangxue Shengfa Capsules was observed with light microscope and image analysis after HE stain.Results The study on the ＂time-toxicity＂ relationship indicated that the hepatotoxicity occurred at 2h after administration and expressed obviously in the range from 6h to 24h after administration.The study on the ＂dose-toxicity＂relationship showed that pathohistological injury of different degree can be induced by drug of（2.5~12.0）g·okg-1 and acidophilia,hyalinization,vacuolus were the main pathologic changes.Conclusion Once oral administration of Yangxue Shengfa Capsules with certain dosage may induce acute hepatotoxical injury in mice and show an obvious ＂dose-time-toxicity＂ relationship.It can cause pathologic changes such as acidophilia,hyalinization,vacuolus to hepatic cells.

关 键 词：养血生发胶囊 肝毒性 病理学 “量-时-毒”关系 

分 类 号：R282[医药卫生—中药学]