养血生发胶囊对小鼠肝毒性“量-时-毒”关系研究  

Experimental Study on the "Dose-Time-Toxicity" Relationship of Acute Hepatotoxicity Induced by Yangxue Shengfa Capsules to Mice

作　　者：李晓宇[1,2] 孙蓉[1] 吕莉莉[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学,山东济南250014

出　　处：《中国药物警戒》2011年第4期204-207,共4页Chinese Journal of Pharmacovigilance

基　　金：国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802);山东省科技平台建设项目课题(2008GG2NS02021)

摘　　要：目的研究养血生发胶囊致小鼠肝毒性的"量-时-毒"关系。方法 "时-毒"关系研究:取小鼠按不同时间点分组,单次灌胃给予一定剂量养血生发胶囊,观察给药后小鼠毒性反应,分别于药后不同时间检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBI),计算肝、肾、脾、胸腺脏器指数。"量-毒"关系研究:取小鼠按不同剂量分组,单次灌胃给予不同剂量的养血生发胶囊分别于给药后2h按"时-毒"研究方法对小鼠进行相应处理。结果小鼠灌胃较高剂量养血生发胶囊后,血清ALT、AST活力在2h达到高峰,持续时间均约达8h;给药后12h肝脏指数升至最高,药后48h可恢复至正常值。养血生发胶囊剂量在(2.50～12.00)g.kg-1之间、对肝组织产生明显损伤,且随着剂量增大,ALT、AST、TBI升高显著。结论小鼠单次灌胃给予一定剂量的养血生发胶囊可造成急性肝损伤,并呈现一定的"量-时-毒"关系。关于其肝脏损伤的机制有待进一步研究。Objective To study the ＂dose-time-toxicity＂ relationship of hepatotoxicity caused by Yangxue Shengfa capsules to mice.Methods Study on the ＂time-toxicity＂ relationship： Ig Yangxue Shengfa capsules of certain dosage to mice and the changes of the level of ALT,AST,TBI in serum and organ index of liver,spleen and thoracic gland were tested at different time points after administration.Study on the ＂dosage-toxicity＂ relationship： Ig2.00）ml·kg-1 and the level of ALT,AST,TBI increased significantly with the increase of dosage.Conclusion once oral administration of Yangxue Shengfa capsuleswith large dosage may induce acute hepatotoxical injury in mice and show an obvious ＂dosage-time-toxicity＂ relationship and Its hepatic injury mechanism needs further research.

关 键 词：养血生发胶囊 小鼠 肝毒性 “量-时-毒”关系 

分 类 号：R282[医药卫生—中药学]