浙江省1999-2010年乙型流感病毒血凝素和神经氨酸酶基因变化特征分析  

Genetic variation of the hemagglutinin and neuraminidase of influenza B viruses isolated in Zhejiang province during 1999-2010

作　　者：茅海燕[1] 周敏[1] 张严峻[1] 陈寅[1] 徐昌平[1] 李榛[1] 严菊英[1] 卢亦愚[1] 

机构地区：[1]浙江省疾病预防控制中心病毒所,杭州310051

出　　处：《中华流行病学杂志》2011年第4期376-381,共6页Chinese Journal of Epidemiology

基　　金：浙江省自然科学基金（Y2080987）

摘　　要：目的分析1999-2010年浙江省乙型流感病毒主要抗原基因血凝素（HA）和神经氨酸酶（NA）的分子变异特征。方法采集浙江省流感暴发疫情和哨点监测医院的流感样患者呼吸道标本，荧光定量RT-PCR快速检测和病毒分离，选取乙型流感病毒分离代表株进行HA和NA基因测序，采用生物信息学软件分析变异和进化。结果共分析浙江省乙型流感病毒分离株34株，其中Victoria系20株，Yamagata系14株；1999—2010年间Victoria系毒株的HA，基因变异率4．5％，Yamagata系毒株为3．4％；2004年后分离的Victoria系毒株均为基因重配株，HA属于Victoria系，而NA属于Yamagata系；2010年新型甲型H1N1流感流行高峰过后，浙江省仍以乙型流感病毒流行为主，分离株与2009—2010年流感疫苗株B／Brisbane／60／2008接近，与往年乙型流感毒株相比HA和NA发生多个氨基酸位点变异。结论1999-2010年浙江省乙型流感病毒流行株发生明显变异，基因重配和抗原漂移是病毒发生变异的主要机制。Objective To Characterize the genetic diversity of hemagglutinin （HA） and neuraminidase （NA） of influenza B viruses isolated in Zhejiang province during 1999-2010. Methods Respiratory specimens were collected from patients with flu-like syndrome during the influenza outbreaks or from the hospitals which carrying out influenza surveillance project in Zhejiang province. Samples were detected by real-time RT-PCR and isolated for influenza virus. HA1 and NA genes of influenza B virus isolates were amplified and sequenced. Phylogenetic comparison and genetic diversity analysis were performed using the bioinformation software. Results A total of 34 influenza B viruses were evolved in this study including Victoria-like and Yamagata-like strains according to the results of the HI test. The mutation rate of Victoria-like HA1 gene was 4.5% and Yamagata-like HA1 gene was 3.4%, respectively. The Victoria-like influenza B isolates had appeared to be all re-assortants having a Victoria lineage HA and Yamagata lineage NA since 2004. The predominant type of influenza virus isolates in 2010 was also influenza B virus after the H1N1 flu pandemic in Zhejiang province. The isolated strains were antigenieally and genetically similar to B/Brisbane/60/2008-the vaccine strain proposed for 2009-2010. Many differences of HA1 and NA amino acids existed in the current isolates when compared to previous influenza B strains. Conclusion Significant diversity was generated among influenza B virus isolated from 1999 to 2010 in Zhejiang province. Genetic re-assortment and antigenic drift seemed the main evolutionary mechanism on influenza B virus.

关 键 词：乙型流感病毒 血凝素 神经氨酸酶 变异 

分 类 号：R51[医药卫生—内科学]