毛细管气相色谱法测定红药贴膏中4种活性成分的含量  

Determination of Four Active Components in Hongyao Strapping by Capillary Gas Chromatography

作　　者：张一鸣[1] 王洁[1] 胡明勋[1] 郝增燕[1] 陈安家[1] 

机构地区：[1]山西医科大学药学院,太原030001

出　　处：《中国实验方剂学杂志》2011年第7期47-50,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山西省自然科学基金项目(2008011077-1)

摘　　要：目的:建立毛细管气相色谱法同时测定红药贴膏中樟脑、薄荷脑、冰片和水杨酸甲酯的含量。方法:采用毛细管气相色谱法。色谱柱为PEG-20M石英毛细管柱(0.25 mm×0.25μm×30 m),氢火焰离子检测器,检测器和气化室的温度均为200℃,柱温160℃,氮气为载气,正辛醇为内标物,用挥发油测定器蒸馏制备供试品溶液。结果:在该色谱条件下,樟脑、薄荷脑、冰片和水杨酸甲酯分离度和线性关系良好,回收率分别为99.8%,100.3%,99.5%,99.7%。结论:该方法简便、准确、专属性强、重复性好,可用于红药贴膏的质量控制。Objective： To establish a method for determination of camphor,menthol,borneol and methylsalicylate in Hongyao strapping.Method：Capillary gas chromatography was adopted.The chromatography separation was carried out with PEG-20M capillary column（0.25 μm × 0.25 mm × 30 m）,using a flame ionization detector.The injector and detector temperature were set at 200 ℃.The column temperature was 160 ℃.n-Octanol was used as internal standard.The sample solution was prepared by distillation with essential oil collector.Result： The resolution and linearity of camphor,menthol,borneol and methylsalicylate were good under the chromatographic conditions.The average recoveries of camphor,menthol,borneol and methylsalicylate were 99.8%,100.3%,99.5%,99.7%.Conclusion：This method is simple,sensitive,accurate and has good repeatability for controlling the quality of camphor,menthol,borneol and methylsalicylate in Hongyao strapping.

关 键 词：毛细管气相色谱法 红药贴膏 樟脑 薄荷脑 冰片 水杨酸甲酯 含量测定 

分 类 号：R284.1[医药卫生—中药学]