HPLC测定芩翘抗感颗粒中黄芩苷的含量  

Determination of Baicalin in Qinqiao Kanggan Granules by HPLC

作　　者：费超[1] 陈然 曹杰[1] 张海鸣[1] 方睿[1] 刘子沐[1] 杜树山[1] 

机构地区：[1]中药资源保护与利用北京市重点实验室,北京100875 [2]北京超微生物制品有限责任公司,北京100007

出　　处：《中国实验方剂学杂志》2011年第7期63-65,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市新医药学科群重点支持项目(xk100270569)

摘　　要：目的:建立芩翘抗感颗粒中黄芩苷的高效液相含量测定方法。方法:采用Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-0.1%磷酸溶液(48∶52)为流动相,流速1 mL.min-1,检测波长280 nm,柱温30℃。结果:黄芩苷含量测定的线性范围0.49～2.46μg(r=0.999 9),精密度试验RSD 0.25%,平均加样回收率100.54%,RSD 1.89%。结论:方法快速、简便、准确、重复性较好,结果可靠,可为芩翘抗感颗粒的质量评价提供有效手段。Objective：To estalish an accurate method for the determination of baicalin in Qinqiao Kanggan granules.Method： Diamonsil C18（4.6 mm × 250 mm,5 μm） column in an oven at 30 ℃ was need,with a mobile phase consisting of mathanol-0.1% phosphoric acid（48∶ 52） and a UV detector at 280 nm,the flow rate was 1.0 mL.min-1.Result： The linear ranges of baicalin was 0.49-2.46μg（r = 0.999 9）.The RSD of measurement precision test was 0.25%;The average recovery was 100.54%（RSD 1.89%,n = 5）.Conclusion： The method was simple and accurate,and can be used for the quality control of Qinqiao Kanggan granules.

关 键 词：芩翘抗感颗粒 黄芩苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]