橡胶树花药愈伤组织继代培养与胚状体发生能力研究  

Anther Callus Culture and Somatic Embryogenesis Abilities in Hevea brasiliensis

作　　者：梁国平[1] 黄凤翔[1] 管艳[1] 李玲[1] 肖三元[1] 

机构地区：[1]云南省热带作物科学研究所,云南景洪666100

出　　处：《热带农业科技》2011年第1期1-3,共3页Tropical Agricultural Science & Technology

基　　金：云南农垦重点科技计划项目(2006XJ03);农业部国家科技支撑计划(2006BAD01AO6-3);云南省产业重大关键技术开发专项计划资助

摘　　要：利用橡胶树花药愈伤组织进行继代培养与胚状体诱导,结果表明,在诱导愈伤组织、继代培养和分化胚状体中MS培养基稍优于改良MS培养基;以继代1代的愈伤组织胚状体的诱导率最高,分别达到70.8%和67.5%;在继代0代、2代和3代之间差异达到极显著,在继代4代时差异达到显著,其他继代次数之间差异不显著。在继代8代后,两种培养基的诱导率逐渐升高,分别达到44.6%和35.3%。In vitro tissue culture and embryogenesis induction of Hevea brasiliensis have revealed that MS medium is not less superior to development MS medium, which both result in the highest induced rate after subcuhuring the calli once （up to 70.8% and 67.5% respectively）. And there are significantly differences in induced rates after subcuhuring the calli zero, twice and third time, slight difference after the fourth time. Whereas the others show no difference, the induced rate would increase gradually for the two media after the eighth time, would reach to 44.6% and 35.3% respectively.

关 键 词：橡胶树 花药培养 愈伤组织 继代培养 胚状体 

分 类 号：S794.105[农业科学—林木遗传育种]