婴幼儿轮状病毒两种检测方法的比较  

The comparison of two methods of infant rotavirus detection

作　　者：孙安民 马春燕 李相磊 

机构地区：[1]河南省开封市第一人民医院检验科,河南开封475000

出　　处：《中国医药导报》2011年第10期91-92,共2页China Medical Herald

摘　　要：目的:比较轮状病毒(RV)金标免疫层析法(GICA)和电镜法(EM),为实验室合理选择检测方法提供依据。方法:采用GICA和EM法对120例婴幼儿腹泻标本和100例正常婴幼儿粪便标本进行检测,计算阳性率,并对其检测结果进行统计学分析与比较。结果:EM法的阳性率37.5%(45/120)高于(GICA)法的阳性率33.3%(40/120),差异有统计学意义(Kappa=0.392,χ2=19.64,P<0.01)。两种方法对正常对照组进行检测,结果均为阴性。结论:GICA法和EM法检测婴幼儿粪便中轮状病毒,结果有明显差异。EM法阳性率高,而GICA法低,并且两种方法的假阳性率几乎不存在。Objective：To carry out the gold standard immunochromatography assay（GICA） and the electron microscopy（EM） to compare the infant rotavirus（RV）,which provides basis for a reasonable detecting method for laboratory.Methods：By using the GICA and the EM methods we selected 120 cases of diarrhea in infants and 100 normal samples of stool specimens for testing,and calculated the positive rates.The results were statistically analyzed and compared.Results：The positive rate by EM method（45/120,37.5%） was higher than that by GICA method（40/120,33.3%）.The differences were significant（Kappa=0.392,χ2=19.64,P〈0.01）.We also detected the normal control group by these two methods,the results were negative.Conclusion：By the GICA and the EM methods to detect the rotavirus in infants,the results are significantly different.The positive rate of EM is high and of the GICA is low.And the false positive rate of the two methods is almost non-existent.

关 键 词：轮状病毒 金标免疫层析法 电镜法 方法比较 

分 类 号：R446.5[医药卫生—诊断学]