用原生质体融合进行冬菇育种的研究  

Studies on the Breeding of Mushrooms by Means of Inactivated Protoplast Fusion

作　　者：刘作喜[1] 郝振军 刘威 许庆兰[1] 赵华[2] 王永吉 

机构地区：[1]黑龙江大学生物工程系,哈尔滨150080 [2]哈尔滨工业大学,哈尔滨150080 [3]黑龙江省自然资源研究所,哈尔滨150040

出　　处：《中国食用菌》1999年第5期6-7,12,共3页Edible Fungi of China

摘　　要：用灭活的〔Flammulinasubnudus (Pr .)Sing〕双核菌株原生质体与〔Flammulinavelutipes (Fr.)Sing〕双核菌株原生质体融合 ,在 2 2℃的条件下筛选得融合子。融合频率为 0× 10 -5～ 4 1× 10 -5。融合子与双亲有明显的拮抗性。融合子的菌丝体形态 ,氨基酸含量 ,子实体的形态 ,以及酸性磷酸酶的测定都与双亲不同。Fusion strains have been obtained by means of high temperature (30℃)selection after fusion between the inactivated protoplasts of Flummulima subnudus (Fr.) Sing dicargons and living protoplasts of Flammulina velutipes(Fr.)Sing, dicargons Fusion among fusion strains snd their parents morphology and amino acid content of hypha, and fruiting bodies morphology of fusion strains are different from of their parents.

关 键 词：近裸冬菇 冬菇 灭活原生质体 融合 育种 

分 类 号：S646.190.3[农业科学—蔬菜学]