β纤维蛋白原基因启动子区HaeⅢ多态性和血浆纤维蛋白原浓度与冠心病的相关性研究  

The association between β HaeⅢ polymorphisms located in the promoter region of β fibrinogen gene, plasma fibrinogen levels and coronary heart disease

作　　者：马会利[1] 陈纪林[1] 

机构地区：[1]中国医学科学院

出　　处：《中华心血管病杂志》1999年第4期277-279,共3页Chinese Journal of Cardiology

摘　　要：目的　研究β纤维蛋白原基因启动子区HaeⅢ多态性 (H1H1、H1H2、H2H2 )和血浆纤维蛋白原浓度与冠心病 (CHD)之间的关系。方法　对 66例CHD患者和 5 3例健康对照者分别进行 βHaeⅢ多态性分析和血浆纤维蛋白原浓度测定。抽提DNA采用酚、氯仿方法 ,血浆纤维蛋白原浓度测定采用Follin酚法。结果　 ( 1)CHD患者携带H1H2 ,H2H2基因型频率较正常对照组明显增高 (P <0 0 1) ;( 2 ) βHaeⅢ多态性与血浆纤维蛋白原浓度间存在显著正相关 (r=0 65 ,P <0 0 0 1) ,以 βHaeⅢ酶切位点缺失的H1H2、H2H2基因型血浆纤维蛋白原浓度明显增高 (P <0 0 1)。结论　CHD患者携带H1H2 ,H2H2基因型频率高 ,血浆纤维蛋白原浓度高 ,是CHD患者动脉血栓形成的主要原因之一。Objective To study the association between plasma fibrinogen levels and the β HaeⅢ polymorphisms located in the promoter rigion of the β fibrinogen gene in patients with coronary heart disease (CHD) Methods β HaeⅢ polymorphisms and plasma fibrinogen levels were simultaneously measured in 66 patients with CHD and 53 control subjects DNA was extracted using the phenol/chloroform method Plasma fibrinogen concentration was assayed with Follin phenol method Results (1) The frequencies of the genotype H1H2, H2H2 were higher in patients with CHD than in control subjects ( P <0 01) (2) β HaeⅢ polymorphisms are significantly associated with plasma fibrinogen levels ( r =0 65, P <0 001) The genotypes (H1H2,H2H2) which lost cutting site for β HaeⅢ enzyme showed that plasma fibrinogen levels were higher in this group ( P <0 01) Conclusion The frequencies of the genotype H1H2, H2H2 were higher in patients with CHD as compared with control subjects and plasma fibrinogen levels in CHD patients were significantly increased It may be one of the major factors of thrombosis in patients with coronary heart disease

关 键 词：冠心病 βHaeⅢ多态性 纤维蛋白原 相关性研究 

分 类 号：R541.4[医药卫生—心血管疾病]