氧化型胆固醇对血管平滑肌细胞的损伤作用  

Cytotoxic effect of oxysterols on cultured rat aortic smooth muscle cells

作　　者：凤志慧[1] 仲崇霞[1] 葛淑君[1] 程时[1] 

机构地区：[1]北京医科大学生物物理系,100083

出　　处：《中华心血管病杂志》1999年第4期300-303,共4页Chinese Journal of Cardiology

基　　金：国家自然科学基金!3 9470 3 44

摘　　要：目的　以胆固醇为对照 ,观察 3 β 5α 6β 三羟胆固烷 (cholestane 3 β,5α,6β triol)、2 5 羟胆固醇 ( 2 5 hydroxycholesterol)、7 酮胆固醇 ( 7 ketocholesterol)及环氧胆固醇 (cholesterol 5α,6α epoxide)四种氧化型胆固醇对大鼠主动脉平滑肌细胞的损伤作用。方法　取 6～ 10代细胞 ,测定细胞存活率、细胞培养液乳酸脱氢酶活力 ,用电子自旋共振自旋标记检测膜脂流动性和膜蛋白构象。结果　氧化型胆固醇呈时间和剂量依赖性降低细胞存活率、增加培养液乳酸脱氢酶活力 ,以 3 β 5α 6β 三羟胆固烷损伤最重。氧化型胆固醇还使膜脂流动性降低、膜蛋白构象改变及运动减慢。胆固醇除改变膜脂流动性外 ,在相同剂量及相同作用时间的情况下 ,对细胞无损伤作用。结论　氧化型胆固醇对血管平滑肌细胞有损伤作用 ,其中以 3 β 5α 6β 三羟胆固烷损伤最重 ,胆固醇对细胞没有损伤作用。氧化型胆固醇的细胞损伤与膜物理性质改变有关。Objective The cytotoxic effects of four oxysterols (cholestane 3β,5α,6β triol,25 hydrocholesterol, 7 ketocholesterol, cholesterol 5α,6α epoxide) on cultured rat aortic smooth muscle cells were compared with that of cholesterol Methods With the cells in passages between 6 and 10,cell viability was detected by trypan blue test Lactate dehydrogenase (LDH) leakage was detected by LDH kit Using electron spin resonance spin labelling, cell membrane lipid fluidity, membrane protein conformation were measured Results Exposed to oxysterols, cell viability decreased and LDH leakage increased in a dose and time dependent manner After being injured by oxysterols membrane lipid fluidity reduced and membrane protein conformation changed significantly Cholesterol in the same dosage and at the same experiment period as oxysterols showed no cytotoxic effect Conclusion (1) Oxysterols are cytotoxic to cultured smooth muscle cell (2) Of these four oxysterols, cholestane 3β,5α,6β triol is the most potent (3)Cholesterol shows no cytotoxicity, except change of membrane lipid fluidity (4) Oxysterol induced cell injury might be associated with change in physical properties of membrane

关 键 词：氧化型胆固醇 血管平滑肌细胞 损伤 

分 类 号：R543[医药卫生—心血管疾病]