EB病毒膜抗原BLLF1基因转基因小鼠的制备  

Generation of Transgenic Mice Canying EB Virus BLLF1 Gene

作　　者：郭长占[1] 周为民[2] 潘秀芳[3] 谷淑燕[2] 田枫[4] 刘斌[3] 王兆绰[4] 高晓明[1] 

机构地区：[1]北京医科大学免疫学系,北京100083 [2]中国预防医学科学院病毒学研究所,北京100052 [3]北京医科大学组织胚胎学系,北京100083 [4]北京医科大学实验动物科学部,北京100083

出　　处：《基础医学与临床》1999年第4期30-32,共3页Basic and Clinical Medicine

摘　　要：将Epstein－Bars病毒（EBV）膜抗原（MA）BLLF1基因用显微注射法导入昆明种小鼠受精卵的雄性原核以制备转基因小鼠模型。共注射受精卵450枚，存活291枚，植入假孕母鼠的输卵管使之发育，产出仔鼠12只。注射后卵的存活率和仔鼠出生率为65％和4％。用PCR法分析显示5只有BLLF1基因整合，整合率为42％。结果表明携带BLLF1基因的转基因小鼠已经制备成功。该转基因小鼠可作为研究EB病毒致病机理，特别是与自身免疫病关系的动物模型。EB Virus is closely associated with human autoimmune disease, such as Sjogren's Syndrome, Rhcumatoid Arthritis.BLLF1 gene encoding membrane antigen (MA) is an actively expressed gene during EB van infection.In order to ivestigate the relationship betWeen EB virus infection and amoimmune disease, we generated transgenic mice carrying EBV BLLF1 gene. PCR analysis indicated that the BLLF1 gene wasintegrated into the genome of mouse cell. This approch is a prelimings attempt to stody the effectsof EBV in autoimmune disease.

关 键 词：转基因小鼠 EB病毒 自身免疫病 膜抗原 

分 类 号：R593.2[医药卫生—内科学]