一氧化氮抑制白介素-1诱导血管内皮细胞粘附分子-1的表达  

Nitric Oxide Inhibits VCAM-1 Expression of Human Endothelial Cell Activated by IL-1

作　　者：霍玉庆[1] 徐成斌[1] 王申五[1] 张杰 张彤[1] 崔俊峰[1] 尹春琳[1] 

机构地区：[1]北京医科大学人民医院心血管内科中心实验室,北京100044 [2]山东泰安中医院

出　　处：《基础医学与临床》1999年第4期38-41,共4页Basic and Clinical Medicine

摘　　要：以硝普钠作为一氧化氮的供体。用流式细胞仪荧光强度测定、细胞ELISA、Northern杂交等方法分别从蛋白表达、基因表达水平研究一氧化氮对内皮细胞粘附分子VCAM-1表达的影响。结果表示：一氧化氮抑制IL－1α诱发的内皮细胞粘附分子VCAM－1的基因表达水平其作用里浓度及时间依赖性。一氧化氮抑制内皮细胞粘附分子VCAM－1的表达是其抑制单核一内皮细胞粘附及抗动脉粥样硬化的机制之一．FCAS, cell ELISA and Nortnern blot were applied to study the effects of nitroprosside, a donorof nitric oxide on the expression of protein and mRNA of VCAM-1. Results showed that nitroprussideinhibit6d Expression of VCAM-1 protein and mRNA in a dose-and time-dependent manner. It impliesthat, inhibitory effect of nitric oxide on VCAM-1 expression could be one of the mechanismsunderlying antiadhesion between monocyte and vascular endothelial cells, and antiatherosclerosis. Correspondence to professor Xu Chengbin

关 键 词：一氧化氮 内皮细胞 血管细胞 粘附分子 IL-1 

分 类 号：R543[医药卫生—心血管疾病]