反义MCP-1转染兔平滑肌细胞及对其生长的影响  

Antisense MCP-1 Gene Transfer with Cationic Lipids into Rabbits AorticSmooth Muscle Cells and its Effect on Cell Proliferation

作　　者：李拥军[1] 管珩[1] 刘佩毛[2] 许漫 赵三妹[2] 王宗立[2] 余铭鹏[2] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院普外科,北京100730 [2]中国医学科学院中国协和医科大学基础医学研究所病理室,北京100005

出　　处：《基础医学与临床》1999年第4期79-84,共6页Basic and Clinical Medicine

摘　　要：构建逆转录病毒载体反义单核细胞趋化蛋白1（LUCX-Anti-MCP－1）表达质粒，利用阳离子脂质体（Lipofectamine）将反义MCP－1导入体外培养的平滑肌细胞，通过PCR、Northern杂交检测外源基因的转染及其mRNA表达情况。应用3H胸腺嘧啶核苷（3H－TdR）掺入法了解反义MCP－1基因对细胞生长的影响．结果显示：Lipofectamine可以成功的将反义MCP－1基因导入体外培养的主动脉平滑肌细胞中，并实现外源基因的转录表达。且反义MCP－1基因对体外培养的平滑肌细胞生长没有影响。DNA can be introduced into a variety of cell types after formation of liposomalcomplexes with cationic lipids. In this report, we constructed an amisense monocyte chemotacticprotein 1 plasmid with retroviral vector (LNCX-Anti-MCP-1), and transferred this gene into smoothmuscle cells of rabbits using cationic lipids (lipofectamine) ex vivo. Genomic DNA PCR tested thecondition of exogenic gene transference. Total RNA Northern hybridization datected whether theexogenic mRNA epression or not. And estimated its effect on cell proliferation with 3H Thymidineincorporation. Results showed that exogenic gene transference and expression were successfill,and the gene had no effect on cell proliferation.

关 键 词：基因转染 反义基因 平滑肌细胞 阳离子脂质体 

分 类 号：R543.02[医药卫生—心血管疾病]