基于油茶组59万条EST序列的转录组学初步分析  

Transcriptome Characterization for Camellia Sect.Oleifera Based on the 592 499 ESTs

作　　者：陈英[1] 江香梅[2] 张露[3] 张新叶[4] 项东云[5] 温强[1,2] 江聪[1] 王光萍[1] 黄敏仁[1] 徐立安[1] 

机构地区：[1]南京林业大学,南京210037 [2]江西省林业科学院,南昌330032 [3]江西农业大学,南昌330045 [4]湖北省林业科学研究院,武汉430079 [5]广西林业科学研究院,南宁530002

出　　处：《林业科学》2011年第2期161-163,共3页Scientia Silvae Sinicae

摘　　要：油茶组（Camellia Sect.Oleifera）植物是我国特有的木本油料植物,主要分布全国14个省（区）,种植面积达300万hm2。茶油为油茶组多个树种种仁油的统称,其中以油茶（Camellia oleifera）最为常见,其次是浙江红山茶（C.chekiangoleosa）和短柱茶（C.brevistyla）等。茶油是优质食用植物油,富含油酸、亚油酸等不饱和脂肪酸,且易消化耐贮藏（何方等,2004）。大幅提高茶油在食用油供给中的比例,可以缓解我国日益紧张的食用油供给矛盾。To investigate gene diversity and expression pattern in Camellia Sect.Oleifera,454 GS FLX platform and Titanium regent were used to produce expressed sequence tags（ESTs） from flower buds of 3 Camellia species.A total of 5 920 499 ESTs were generated.Clustering those sequences twice by Cap3 produced 15 733 contigs and 20 606 singlets in C.oleifera,19 397 contigs and 26 882 singlets in C.chekiangoleosa,14 779 contigs and 25 389 singlets in C.brevistyla.The GC gradients in 3 transcriptomes basically followed a normal distribution.In additional all contigs and singlets were functionally categorized into GO（Gene Ontology） categories respectively.

关 键 词：油茶 转录组 454 GS FLX 表达序列标签 

分 类 号：S718.46[农业科学—林学] Q943.2[生物学—植物学]