新型Gemini表面活性剂G_(14-3-14)与DNA的相互作用及其在共振光散射法测定DNA中的应用  

A new Gemini surfactant(G_(14-3-14))′s interaction with DNA and its application in DNA detection by resonance light scattering

作　　者：盛立娇[1] 潘全[1] 宋功武[1] 周建刚[1] 

机构地区：[1]湖北大学有机功能分子合成与应用教育部重点实验室,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2011年第1期88-93,共6页Journal of Hubei University：Natural Science

基　　金：湖北省自然科学基金项目(2008CDB017);有机功能分子合成与应用教育部重点实验基金项目(2006-KL-006)资助

摘　　要：以芘（pyrene）为荧光探针,应用稳态荧光法探讨阳离子型Gemini表面活性剂G14-3-14与脱氧核糖核酸（DNA）的相互作用,并求得G14-3-14的临界胶束浓度（CMC）为3.8×10^-4mol.L^-1.根据DNA对G14-3-14370nm处共振光散射信号的增强效应,建立以G14-3-14为探针测定DNA的共振光散射法,并对介质酸度、离子强度、表面活性剂浓度及共存物质干扰等实验条件进行优化.在最佳条件下绘制了工作曲线,该方法线性范围为0-3 450μg.L^-1,检出限为28.6μg.L^-1,用于合成样品中DNA的测定,结果令人满意.此外,通过红外光谱及共振光散射光谱初步探讨了G14-3-14与DNA相互作用的机理,即G14-3-14在DNA分子表面进行聚集和长距组装,从而导致G14-3-14共振光散射信号的增强,这也是该方法测定DNA含量的理论基础.The paper investigated the interaction between a new Gemini surfactant G14-3-14 and DNA by applying pyrene as fluorescence reagent.A detection assay was proposed based on the enhanced resonance light scattering（RLS） signals of G14-3-14 at 370 nm by DNA at pH 3.51.The effects of pH,ionic strength,G14-3-14 concentration and coexisting substances on the RLS enhancement were discussed,respectively.The intensity of RLS signal was proportional to the concentration of DNA in the range from 0 to 3 450 μg·L-1 with the determination limit（3σ） of 28.6 μg·L-1.This novel method had been applied to the assay for four synthetic samples with satisfactory results.Infra-red and RLS spectra were used to study the interaction mechanism.The long range assembly of G14-3-14 on the molecular surfaces of DNA occurred with electrostatic and hydrophobic interaction,which results in enhanced resonance light scattering（RLS） signals.

关 键 词：GEMINI表面活性剂 共振光散射技术 脱氧核糖核酸 芘探针 红外光谱 

分 类 号：O657.3[理学—分析化学]