辣椒抗根结线虫基因Me3的精细定位  

Fine Mapping of the Root-knot Nematode Resistance Gene Me3 in Pepper

作　　者：许小艳[1,2] 刘峰[1,2] 康厚祥[1] 张竹青[2] 邹学校[2] 谢丙炎[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]湖南省农业科学院蔬菜研究所,长沙410128

出　　处：《园艺学报》2011年第2期288-294,共7页Acta Horticulturae Sinica

基　　金：国家大宗蔬菜产业技术体系项目;公益性行业(农业)科研专项(2011303018)

摘　　要：以含Me3基因的抗根结线虫辣椒自交系‘HDA149’为父本,感根结线虫辣椒自交系‘8214’为母本,构建了一个2133株的F2作图群体。采用群体分离分析法(Bulked Segregant Analysis,BSA),构建抗、感病两个DNA池,对由两亲本筛选出来的285对引物进行扩增分析,发现引物SSCP_B322和EPMS658在抗、感池间具有稳定的多态性。继续用这两对引物对F2单株进行扩增,以JoinMap 3.0软件分析发现SSCP_B322和EPMS658标记位于Me3基因两侧,遗传图距分别为0.56cM和1.33cM。运用回交群体BC1和F3群体验证实了这两个标记,可有效用于辣椒抗线虫分子标记辅助育种,也为图位克隆Me3基因奠定了基础。The pepper（Capsicum annuum）resistant inbred line ＇HDA149＇ with Me3 was used as male parent,and the susceptible inbred line ＇8214＇ was used as female parent in the study.Mapping population included 2 133 F2 individuals.Bulked-segregant analysis with DNA pools,from susceptible or resistant F2 progenies,was performed to identify markers linked to Me3.There were 285 pairs of primers with polymorphism between two parents,but just SSCP_B322 and EPMS658 were polymorphic between the two bulks.Then F2 individuals were amplified with the two pairs of primers,recording data.Linkage analysis used JoinMap 3.0 software.The genetic distance of SSCP_B322 and EPMS658 was 0.56 cM and 1.33 cM respectively,and two markers were two sides of Me3 gene.They had been verified in BC1 group and F3 families,which has been practically useful to marker-assisted selection in pepper breeding and Me3 gene cloning.

关 键 词：辣椒 根结线虫 Me3基因 精细定位 

分 类 号：S641.3[农业科学—蔬菜学]