春兰×大花蕙兰杂种AGL6同源基因的克隆及其功能研究  

Cloning and Functional Research of an AGL6 Homologous Gene in Hybird of Cymbidium goeringii and Cymbidium hybirdium

作　　者：胡立霞[1] 徐京[1] 庞基良[1] 王利琳[1] 王慧中[1] 余红[2] 

机构地区：[1]杭州师范大学生命与环境科学学院,杭州310036 [2]杭州市农业科学研究院生物技术研究所,杭州310024

出　　处：《园艺学报》2011年第2期317-326,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31071818);杭州市科学技术局项目(20070232H08;20080432T06)

摘　　要：以春兰×大花蕙兰杂种试管苗形成的花蕾为材料,利用RACE技术克隆到AGL6基因的全长cDNA,命名为CgAGL6(在GenBank中登录号为GQ265900)。序列分析表明,CgAGL6包含一个完整的ORF,长度为729bp,编码242个氨基酸;含有AGL6基因所共有的2个保守结构域MADS-box和K-box。通过蛋白序列比对,发现CgAGL6与玉米的ZmZAG3、水稻的OsMADS6有70%的相似性,与拟南芥的AtAGL6有61%的相似性。蛋白质同源性分析表明CgAGL6属于AGL6分支中单子叶植物的分类群。将CgAGL6置于CaMV35S启动子控制下在烟草中异位表达,转基因烟草表现出开花时间提前,雄蕊部分花瓣化,花粉发育不良和花瓣数增多或减少等新表型,表明CgAGL6参与了转基因烟草开花时间的调控及花器官的发育。Full-length cDNA of CgAGL6（GenBank accession number GQ265900）was cloned from the floral bud of hybird of Cymbidium goeringii and C.hybirdium by rapid amplification of cDNA ends（RACE）technology.The cDNA sequence harbored an open reading frame（ORF）encoding 242 amino acids.Bioinformatics analysis indicated that CgAGL6 has two conserved domains as other AGL6 proteins：MADS-box and K-box.CgAGL6 are similar to GenBank accession L46397（Zea mays）,U78782（Oryza sativa）and M55554（Arabidopsis thaliana）with homology of 70%,70% and 61% respectively.Transgenic tobacco plants ectopically expressing CgAGL6 exhibited novel phenotypes with plant size reduced significantly,flowering early,widely homeotic conversion of stamens into petal-like structures,hypogenesis of pollen,number addition or reduction of petals.These results indicated that CgAGL6 was involved in the regulation of flower transition and flower organ development.

关 键 词：兰属 春兰×大花蕙兰 AGL6同源基因 花发育 

分 类 号：S682.31[农业科学—观赏园艺]