超声微泡靶向增强RNA干扰质粒转染对移植瘤微血管形成和细胞凋亡的影响  

Affects of Microvessel and Cell Apoptosis by UTMD Associated with Short Hairpin RNA Plasmid Technique to Silence Survivin Expression in vivo

作　　者：陈智毅[1] 梁琨[2] 邱日想[3] 袁文琳[1] 

机构地区：[1]广州医学院第三附属医院医学超声科,广州市510150 [2]广州医学院第三附属医院妇产科,广州市510150 [3]广州医学院第三附属医院药剂科,广州市510150

出　　处：《中国超声医学杂志》2011年第3期193-196,共4页Chinese Journal of Ultrasound in Medicine

基　　金：广东省医学科研基金项目(No.A2010270)

摘　　要：目的通过超声微泡靶向破坏的方法,选择Survivin作为靶点对短发夹状RNA干扰质粒(pSIREN/S3)进行转染,探讨其对裸鼠移植瘤微血管形成和细胞凋亡的影响。方法将荷瘤裸鼠分为对照组、超声辐照诱导质粒组及超声微泡靶向破坏诱导质粒组,对组织样本行组织学检查,测定微血管密度(MVD),以末端脱氧核苷酸转移酶标记法(TUNEL)定量检测分析荷瘤裸鼠模型肿瘤组织的凋亡指数(AI)。结果超声微泡靶向破坏诱导质粒组的MVD明显减少,AI明显升高,与对照组及超声辐照诱导质粒组比较,差异均有统计学意义(P<0.05)。结论超声微泡靶向破坏联合pSIREN/S3质粒敲除Survivin,能使肿瘤组织中血管生成减少,明显诱导细胞凋亡,为恶性肿瘤基因治疗领域的一个新方法。Objective To investigate the affects of microvessel and apoptosis induction transfected by ultrasound targeted mierobubble destruction （UTMD） techniques associated with short hairpin RNA plasmid （pSIREN/S3） techniques in transplanted tumors experiment. Methods Nude mice were randomly arranged into 3 groups： control group, plasmid injection and ultrasound （P＋US）, P＋UTMD group. Histological examinations were evaluated. Microvessel density （MVD） was detected by CD34 protein expressions and apoptotic index （AI） was measured by Terminal deoxynucleotidy] transferase-mediated dUTP nick end labeling（TUNEL）. Results Comparing with those in C and P＋ US groups, MVD decreased significantly, whereas AI increased remarkably （P〈0.05）. Conclusions UTMD combined with shRNA technique can induce apoptosis and reduce angiogenesis without causing any apparently adverse effect, representing a new technology in cancer gene therapy.

关 键 词：超声 微泡 凋亡 转染 基因治疗 

分 类 号：R445.1[医药卫生—影像医学与核医学] R730.5[医药卫生—诊断学]