过氧化氢酶基因-262C／T及-21A／T多态性与燃煤型氟中毒相关性的研究  

Relationship between -262C/T and -21A/T polymorphism of catalase gene and coal-burning borne fluorosis

作　　者：涂茜[1] 单可人[1] 张婷[1] 何燕[1] 徐仕清 周曙光 王婵娟[1] 吴昌学[1] 李毅[1] 官志忠[1] 

机构地区：[1]贵阳医学院分子生物学重点实验室,550004 [2]贵州省毕节市疾病预防控制中心 [3]贵州省长顺县疾病预防控制中心

出　　处：《中国地方病学杂志》2011年第2期148-151,共4页Chinese Jouranl of Endemiology

摘　　要：目的探讨过氧化氢酶（CAT）基因启动子区-262C／T、-21A／T多态性与燃煤型氟中毒之间的关系。方法2007年，在贵州省毕节市鸭池镇燃煤型氟中毒非改灶村抽取150名村民作为非干预组，于该市长春镇燃煤型氟中毒改灶村抽取150名村民作为干预组，于非地方性氟中毒长顺县白云山镇抽取150名村民作对照组，应用聚合酶链反应限制性片段长度多态性（PCR—RFLP）分析方法，分别检测各组CAT-262C／T、-21A／T多态的基因型。结果非干预组、干预组和对照组CAT-262C／r、CAT-21A／T多态的基因型频率分布符合Hardy—weinberg遗传平衡定律（P〉0．05）。在研究人群中检测到CAT-262C／T多态的两种基因型CC和CT，未检测到TT基因型：检测到CAT-21A／T多态的三种基因型AA、AT和TT。对照组、干预组和非干预组CAT基因—262C／T多态位点的基因型（CC、CT）频率分别为89．33％（134／150）、10．67％（16／150）、88．67％（133／150）、11．33％（17／150）、93．33％（140／150）、6．67％（10／150）；对照组、干预组和非干预组C等位基因频率分别为94．67％（284／300）、94.33％（283／300）、96．67％（290／300）；T等位基因频率分别为5-33％（16／300）、5．67％（17／300）、3．33％（10／300）。对照组，干预组和非干预组CAT基因一21A／T多态位点基因型（AA、AT、TT）频率分别为48．67％（73／150）、46．00％（69／150）、5.33％（8／150）、52．67％（79／150）、38．00％（57／150）、9．33％（14／150）、51.33％（77／150）、38．00％（57／150）、10．67％（16／150）；对照组、干预组和非干预组A等位基因频率分别为71．67％（215／300）、71．67％（215／300）、70．33％（211／300）；T等位基因频率分别为28_33％（85／300）、28．33％（85／300）、29．67％（89／300）。CAT-262C／T和CAT-21A/T基因型频率及等位基因频率在对�Objective To explore the relationship between -262C/T and -21A/T polymorphisms of eatalase（CAT） gene and coal-burning borne fluorosis. Methods In 2007, 150 villagers were taken as a non- intervention group in Bijie city from the village of coal-burning borne fluorosis areas with unchanged cooking stoves ; 150 villagers were taken as the intervention group from the town of Changchun county where cooking stoves changed; 150 villagers were taken as control from non-endemic fluorosis areas in Baiyun town of Changshun county. PCR-restriction fragment length polymorphism were employed to detect genotypes of CAT-262C/T and CAT-21A/T polymorphism of CAT gene. Results The genotypic frequencies of CAT-262C/T and CAT-21A/T in non- intervention group,intervention group and control group were in line with Hardy-Weinberg equilibrium law（P 〉 0.05 ）. The genotypes of CC and CT were detected while no TT were detected for CAT-262C/T polyinorphism; the genotypes of AA, AT and TT were detected for CAT-21A/T. The genotype frequencies of CAT-262 CC, CT in control group, intervention group and non-intervention group were （89.33% （134/150）, 10.67%（16/150）; 88.67% （133/150）, 11.33% （17/150）,93.33% （140/150）,6.67% （10/150）, respectively. The gene frequency of C in control group, intervention group and non-intervention group were （94.67% （284/300）, 94.33% （283/300）, 96.67% （290/300）, respectively. The gene frequency of T in control group, intervention group and non-intervention group were 5.33% （16/300）, 5.67% （17/300）, 3.33% （10/300）, respectively. The genotype frequencies of CAT-21 AA, AT and TF in control group, intervention group and non-intervention group were 48.67% （73/150）,46.00% （69/150）, 5.33% （8/150）, 52.67% （79/150）, 38.00% （57/150）, 9.33% （14/150）, 51.33% （77/150）, 38.00% （57/150）, 10.67% （16/150）, respectively. The gene frequency of A in control group, intervention group and non-intervention group were 71.67% （215/300）,71.67%

关 键 词：氟化物 煤 过氧化氢酶 多态性 单核苷酸 

分 类 号：R5[医药卫生—内科学]