西洋参皂甙对免疫抑制小鼠免疫功能的影响  

Effect of Panax Ginsenoside on Immunological Function of Immuno-suppressive Mice

作　　者：赵云利[1] 吴华彰[2] 杨晶[1] 张杰[1] 周纯先[1] 

机构地区：[1]蚌埠医学院预防医学系,安徽蚌埠233030 [2]蚌埠医学院生物科学系,安徽蚌埠233030

出　　处：《中国生物制品学杂志》2011年第3期305-308,312,共5页Chinese Journal of Biologicals

基　　金：蚌埠医学院自然科学项目(BY0751)

摘　　要：目的探讨西洋参皂甙对免疫抑制小鼠免疫功能的影响。方法用不同浓度的西洋参皂甙[0.12、1.20和12 g/(kg.d)]灌胃小鼠,每天1次,连续灌胃21 d,第10～14天,经腹腔注射环磷酰胺0.1 g/(kg.d)。测定小鼠血中血小板(PLT)、白细胞(WBC)、红细胞(RBC)的数量和血红蛋白(Hb)含量以及免疫器官重量;鸡红细胞吞噬试验检测小鼠腹腔巨噬细胞的吞噬功能,并进行二硝基氟苯(2,4-D)皮肤试验(DTH);体外测定小鼠腹腔巨噬细胞和脾淋巴细胞的增殖活性、腹腔巨噬细胞NO含量以及脾淋巴细胞转化功能。结果西洋参皂甙可使免疫抑制小鼠血中的PLT、WBC、RBC和Hb的降低恢复良好,并恢复小鼠免疫器官重量,2,4-D所致迟发型超敏反应减轻,促进小鼠腹腔巨噬细胞代谢,增强巨噬细胞吞噬功能,诱导巨噬细胞产生NO,提高脾淋巴细胞转化率及淋转指数。结论西洋参皂甙能提高免疫抑制小鼠巨噬细胞吞噬功能,增强小鼠细胞免疫与体液免疫功能。Objective To investigate the effect of Panax Ginsenoside on immunological function of immune-suppressive mice.Methods Mice were administrated with Panax Ginsenoside at various concentrations [0.12,1.20 and 12 g /（kg·d）] by gavage respectively,once a day for 21 d,and injected i.p.with cyclophosphamide at a dosage of 0.1 g /（kg·d） on days 10 ～ 14,then counted for platelet（PLT）,white blood cell（WBC） and red blood cell（RBC） in sera,determined for the content of hemoglobin（Hb） and the weights of immune organs.The mice were also determined for phagocytic function of celiac macrophages by chick erythrocyte phagocytosis test and subjected to DTH skin test with 2,4-D.The proliferative activities of celiac macrophages and splenic lymphocytes,the nitric oxide（NO） content in celiac macrophages and the splenic lymphocyte transformation function of the mice were determined in vitro.Results Panax Ginsenoside recovered the decreases of PLT,WBC,RBC and Hb in sera as well as the weights of immune organs,relieved the DTH caused by 2,4-D,promoted the metabolism,enhanced the phagocytic function and induced the NO production of celiac macrophages,and increased the splenic lymphocyte transformation rate and index.Conclusion Panax Ginsenoside enhanced the phagocytic function of macrophages as well as cellular and humoral immunities of immune-suppressive mice.

关 键 词：西洋参 皂甙类 免疫抑制 免疫功能 

分 类 号：R979.5[医药卫生—药品]