嗜水气单胞菌J-1株灭活疫苗生产工艺的改进  

Improvement of Production Procedure for Inactivated Vaccine against Aeromonas hydrophila J-1 Strain

作　　者：陶家发[1] 赖迎迢[1] 任燕[1] 江小燕[1] 康光辉[1] 张悠[1] 石存斌[1] 吴淑勤[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所广东省水产动物免疫技术重点实验室,广州510380

出　　处：《中国生物制品学杂志》2011年第3期345-348,共4页Chinese Journal of Biologicals

基　　金：农业公益性行业科研专项(200803013);农业科技成果转化资金项目(2008GB23260393)

摘　　要：目的改进嗜水气单胞菌J-1株灭活疫苗的生产工艺,提高培养效率。方法采用改良培养基和产毒素培养基,利用机械式通风搅拌培养J-1菌株,确定发酵培养时间,并比较两种培养基的培养效果;用摇瓶灭活和罐灭活J-1菌株培养液,比较不同浓度甲醛的灭活效果。结果用机械搅拌发酵罐培养J-1菌株,菌体生长12～14 h达峰值,产毒素培养基菌液活菌数平均为102×108 CFU/ml,改良培养基活菌数平均为216×108 CFU/ml,较产毒素培养基提高了111.7%,活菌数最大可达300×108 CFU/ml;改良培养基培养J-1菌株,培养液毒力略高于产毒素培养基;0.3%的福尔马林于37℃灭活24 h,可完全灭活J-1菌株培养液,且制备的灭活疫苗福尔马林残留量符合质量标准。结论改良培养基培养J-1菌株的效果明显优于产毒素培养基,机械式搅拌发酵罐培养嗜水气单胞菌,可比原工艺缩短培养时间一半。Objective To improve the production procedure for inactivated vaccine against Aeromonas hydrophila J-1 strain and increase the culture efficiency.Methods J-1 strain was cultured in modified and toxin-producing media by mechanical stirring under ventilating condition,based on which the time for fermentation was determined,and the culture effets of the two media were compared.The culture liquid of J-1 strain was inactivated in shake-flask and in inactivation tank respectively,and the inactivation effects by formaldehyde at various concentrations were compared.Results The growth level of J-1 strain reached a peak value 12 ～ 24 h after culture in fermentation tank by mechanical stirring.The mean viable count in culture liquid in modified medium was 216 × 108 CFU / ml,which increased by 111.7% as compared with that in toxin-producing medium（102 × 108 CFU / ml）,and reached 300 × 108 CFU / ml at most.However,the virulence of culture liquid in modified medium was slightly higher than that in toxin-producing medium.The treatment with 0.3% formalin at 37℃ for 24 h inactivated the culture liquid completely,while the residual formalin content in prepared inactivated vaccine met the relevant quality standard.Conclusion Modified medium was superior to toxin-producing medium in culture of J-1 strain,and the time for culture of A.hydrophia by mechanical stirring in fermentation tank was only a half of that by original procedure.

关 键 词：嗜水气单胞菌 疫苗 灭活 生产工艺 

分 类 号：R373.2[医药卫生—病原生物学] Q78[医药卫生—基础医学]