Comparative study on modeccin- and phytohemagglutinin （PHA）-induced secretion of cytokines and nitric oxide （NO） in RAW264.7 cells  

作　　者：Daekyung Kim Yasuhiro Yamasaki Zedong Jiang Yuki Nakayama Tomohiro Yamanishi Kenichi Yamaguchi Tatsuya Oda 

机构地区：[1]Jeju Center, Korea Basic Science Institute (KBSI), 66 Jejudaehakno, Jeju-Si, Jeju Special Self-Governing Province 690-756, South Korea [2]Division of Biochemistry, Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan

出　　处：《Acta Biochimica et Biophysica Sinica》2011年第1期52-60,共9页生物化学与生物物理学报（英文版）

摘　　要：The effects of cytotoxic lectins, modeccin and phytohe- magglutinin （PHA） on mouse macrophage cell line RAW264.7 was studied by detecting the induction of inflammatory mediators. Results showed that modeccin induced the release of tumor necrosis factor-α （TNF-α from RAW264.7 cells with a bell-shape concentrationdependent profile. PHA that showed no significant cytotoxicity on RAW264.7 cells up to 100,000 ng/ml induced much higher level of TNF-α than modeccin. PHA simultaneously induced the secretion of granulocyte colony stimulation factor （G-CSF） from RAW264.7 cells with even much higher level than that of TNF-α, whereas modeccin did not. Furthermore, PHA induced the secretion of nitric oxide （NO） in RAW264.7 cells, while no significant level of NO was detected in the modeccin-treated cells. NH4Cl （a lysomotoropic agent） and cycloheximide （a ribosome inhibitor） strongly inhibited modeccin-induced TNF-α secretion, but no significant inhibitory effects of these reagents on the PHA-induced TNF-α secretion were observed. Contrary to modeccin-induced TNF-α secretion, even slightly increased TNF-α secretion was observed in PHA-treated cells in the presence of 10 mM NH4Cl. In addition, the inhibition profiles of modeccininduced TNF-α secretion by various kinase inhibitors were different from those of PHA. These results suggested that the action mode of modeccin to stimulate RAW264.7 cells leading to the secretion of inflammatory molecules, including TNF-α, is distinct from that of PHA. On the other hand, significantly increased translocation of activator protein-1 （AP-1）, a crucial transcription factor involved in expression of inflammatory molecules, into nucleus was observed in RAW264.7 cells treated with PHA and modeccin.The effects of cytotoxic lectins, modeccin and phytohe- magglutinin （PHA） on mouse macrophage cell line RAW264.7 was studied by detecting the induction of inflammatory mediators. Results showed that modeccin induced the release of tumor necrosis factor-α （TNF-α from RAW264.7 cells with a bell-shape concentrationdependent profile. PHA that showed no significant cytotoxicity on RAW264.7 cells up to 100,000 ng/ml induced much higher level of TNF-α than modeccin. PHA simultaneously induced the secretion of granulocyte colony stimulation factor （G-CSF） from RAW264.7 cells with even much higher level than that of TNF-α, whereas modeccin did not. Furthermore, PHA induced the secretion of nitric oxide （NO） in RAW264.7 cells, while no significant level of NO was detected in the modeccin-treated cells. NH4Cl （a lysomotoropic agent） and cycloheximide （a ribosome inhibitor） strongly inhibited modeccin-induced TNF-α secretion, but no significant inhibitory effects of these reagents on the PHA-induced TNF-α secretion were observed. Contrary to modeccin-induced TNF-α secretion, even slightly increased TNF-α secretion was observed in PHA-treated cells in the presence of 10 mM NH4Cl. In addition, the inhibition profiles of modeccininduced TNF-α secretion by various kinase inhibitors were different from those of PHA. These results suggested that the action mode of modeccin to stimulate RAW264.7 cells leading to the secretion of inflammatory molecules, including TNF-α, is distinct from that of PHA. On the other hand, significantly increased translocation of activator protein-1 （AP-1）, a crucial transcription factor involved in expression of inflammatory molecules, into nucleus was observed in RAW264.7 cells treated with PHA and modeccin.

关 键 词：modeccin PHA TNF-α G-CSF nitric oxide 

分 类 号：Q26[生物学—细胞生物学] S965.116[农业科学—水产养殖]