Human mesenchymal stem cells are sensitive to abnormal gravity and exhibit classic apoptotic features  

作　　者：Rui Meng Hui-yun Xu Sheng-meng Di Dong-yan Shi Ai-rong Qian Jin-fu Wang Peng Shang 

机构地区：[1]Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Faculty of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China [2]Institute of Cell Biology and Genetics, College of Life Science, Zhejiang University, Hangzhou 310058, China

出　　处：《Acta Biochimica et Biophysica Sinica》2011年第2期133-142,共10页生物化学与生物物理学报（英文版）

基　　金：Acknowledgements The authors thank Dr. Dan Hospital of Zhejiang Province Shen in the First people＇s for providing human BM.This study was supported by the grant from the National Nature Science Foundation of China （No. 30970689）.

摘　　要：The aim of the present study was to investigate the effects of abnormal gravity on human mesenchymal stem cells （hMSCs）. Strong magnetic field and magnetic field gradient generate a magnetic force that can add to or subtract from the gravitational force. In this study, this is defined as a high-magneto-gravitational environment （HMGE）. The HMGE provides three apparent gravity levels, i.e. hypogravity （μg）, hypergravity （2g） and normal gravity with strong magnetic field （1g） conditions. After hMSCs were subject to HMGE for 12 h, the proliferation, morphology, structure and apoptosis were investigated. Results showed that the proliferation of hMSCs was inhibited under μg condition. The abnormal gravity induced morphologic characteristics of apoptosis cells, such as cell shrinkage, membrane blebbing, nuclear chromatin con- densation and margination, decreased cell viability, and increased caspase-3/7 activity. The rate of apoptosis under μg condition is up to 56.95%. The F-actin stress fibers and microtubules were disrupted under abnormal gravity condition. Under μg-condition, the expression of p53 at mRNA and protein levels was up-regulated more than 9- and 6 folds, respectively. The Pifithrin-α, an specific inhibitor of p53, inhibited the apoptosis and prevented the disruption of cytoskeleton induced by abnormal gravity. These results implied that hMSCs were sensitive to abnormal gravity and exhibited classic apoptotic features, which might be associated with p53 signaling.The aim of the present study was to investigate the effects of abnormal gravity on human mesenchymal stem cells （hMSCs）. Strong magnetic field and magnetic field gradient generate a magnetic force that can add to or subtract from the gravitational force. In this study, this is defined as a high-magneto-gravitational environment （HMGE）. The HMGE provides three apparent gravity levels, i.e. hypogravity （μg）, hypergravity （2g） and normal gravity with strong magnetic field （1g） conditions. After hMSCs were subject to HMGE for 12 h, the proliferation, morphology, structure and apoptosis were investigated. Results showed that the proliferation of hMSCs was inhibited under μg condition. The abnormal gravity induced morphologic characteristics of apoptosis cells, such as cell shrinkage, membrane blebbing, nuclear chromatin con- densation and margination, decreased cell viability, and increased caspase-3/7 activity. The rate of apoptosis under μg condition is up to 56.95%. The F-actin stress fibers and microtubules were disrupted under abnormal gravity condition. Under μg-condition, the expression of p53 at mRNA and protein levels was up-regulated more than 9- and 6 folds, respectively. The Pifithrin-α, an specific inhibitor of p53, inhibited the apoptosis and prevented the disruption of cytoskeleton induced by abnormal gravity. These results implied that hMSCs were sensitive to abnormal gravity and exhibited classic apoptotic features, which might be associated with p53 signaling.

关 键 词：human mesenchymal stem cells abnormal gravity sensitivity APOPTOSIS p53 

分 类 号：Q254[生物学—细胞生物学] Q255